Fig. 5

Identification of the causal variant of reduced ethanol production in the SUC2 locus. A Candidate variants in SUC2. B Meiotic crossovers within the SUC2 locus in the F₆ segregants. Swapping the intergenic variant (-6) yields minor phenotypic effect, whereas swapping the true causal variant (394) yields the same major effect as swapping the entire haplotype block. C Ethanol concentration at the end of fermentation (16^oP) of the wild type (WT) strain RM11-1a and YJM975α and the respective variant-swapped mutants. D Ethanol concentration at the end of fermentation (16^oP) of the wild-type strain S. boulardii, Ethanol Red and CEN.PK and the SUC2^394∆ variant mutant. E Interaction network of SUC2 (green node) and genes whose coding sequence are altered by variants that were identified for ethanol concentration phenotype (pink node). The thickness of the edges represents the confidence score associated with the interaction as determined by STRING. Data are shown with mean ± STD; P-values are indicated with the level of significance (ns: not significant, *: p ≤ 0.05, **: p ≤ 0.01, ***: p ≤ 0.001, ****: p ≤ 0.0001)