Table 1 Overview of generated and analyzed transgenic A. thaliana plants
From: Plastidial wax ester biosynthesis as a tool to synthesize shorter and more saturated wax esters
Construct expressed (short name) (βcon/gly: seed-specific promoters, 35S: 35S promoter) | Number of independent lines after herbicide treatment | Number of screened heterozygous lines by TLC (number of lines with increased WE amounts) | Plant lines used for further analysis |
|---|---|---|---|
βcon::YFP-myc-MaFAR gly::CFP-flag-MaWSD2 (MaFAR/MaWSD2) | 18 | T2 seeds: 17 (9) | ‡§lines 2, 6, 17 |
βcon::cl-YFP-myc-MaFAR gly::cl-CFP-flag-MaWSD2 (clMaFAR/clMaWSD2) | 85 | T2 seeds: 36 (22) | ‡§lines 11, 28, 35 |
βcon::YFP-myc-MaFAR gly::CFP-flag-MaWSD5 (MaFAR/MaWSD5) | 52 | T2 seeds: 19 (16) [9 (6) lines screened for [17], 10 (10) additional lines screened for this publication] | ‡§lines 11, 12, 17 (lines 2, 4, 5, 7, 10 [17]) |
βcon::cl-YFP-myc-MaFAR gly::cl-CFP-flag-MaWSD5 (clMaFAR/clMaWSD5) | 43 | T2 seeds: 18 (12) | ‡§lines 4, 12, 18 |
35S::cl-YFP-myc-MaFAR 35S::cl-CFP-flag-MaWSD2 (35S::clMaFAR/35S::clMaWSD2) | 72 | T2 seeds: 22 (0) T1 leaves: 5 (0) | †line 21 |
35S::cl-YFP-myc-MaFAR 35S::cl-CFP-flag-MaWSD5 (35S::clMaFAR/35S::clMaWSD5) | 56 | T2 seeds: 23 (0) T1 leaves: 9 (0) | †lines 18, 19, 23 |
gly::cl-CFP-flag-MaWSD2 | 100 | T2 seeds: 21 (0) | Â |
gly::cl-CFP-flag-MaWSD5 | 95 | T2 seeds: 21 (0) | Â |
35S::cl-CFP-flag-MaWSD2 | 50 | T2 seeds: 12 (0) T1 leaves: 5 (0) | Â |
35S::cl-CFP-flag-MaWSD5 | 91 | T2 seeds: 21 (0) T1 leaves: 38 (0) | Â |