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Table 1 Overview of generated and analyzed transgenic A. thaliana plants

From: Plastidial wax ester biosynthesis as a tool to synthesize shorter and more saturated wax esters

Construct expressed (short name)
(βcon/gly: seed-specific promoters, 35S: 35S promoter)
Number of independent lines after herbicide treatment Number of screened heterozygous lines by TLC (number of lines with increased WE amounts) Plant lines used for further analysis
βcon::YFP-myc-MaFAR
gly::CFP-flag-MaWSD2
(MaFAR/MaWSD2)
18 T2 seeds: 17 (9) ‡§lines 2, 6, 17
βcon::cl-YFP-myc-MaFAR
gly::cl-CFP-flag-MaWSD2
(clMaFAR/clMaWSD2)
85 T2 seeds: 36 (22) ‡§lines 11, 28, 35
βcon::YFP-myc-MaFAR
gly::CFP-flag-MaWSD5
(MaFAR/MaWSD5)
52 T2 seeds: 19 (16)
[9 (6) lines screened for [17], 10 (10) additional lines screened for this publication]
‡§lines 11, 12, 17
(lines 2, 4, 5, 7, 10 [17])
βcon::cl-YFP-myc-MaFAR
gly::cl-CFP-flag-MaWSD5
(clMaFAR/clMaWSD5)
43 T2 seeds: 18 (12) ‡§lines 4, 12, 18
35S::cl-YFP-myc-MaFAR
35S::cl-CFP-flag-MaWSD2
(35S::clMaFAR/35S::clMaWSD2)
72 T2 seeds: 22 (0)
T1 leaves: 5 (0)
line 21
35S::cl-YFP-myc-MaFAR
35S::cl-CFP-flag-MaWSD5
(35S::clMaFAR/35S::clMaWSD5)
56 T2 seeds: 23 (0)
T1 leaves: 9 (0)
lines 18, 19, 23
gly::cl-CFP-flag-MaWSD2 100 T2 seeds: 21 (0)  
gly::cl-CFP-flag-MaWSD5 95 T2 seeds: 21 (0)  
35S::cl-CFP-flag-MaWSD2 50 T2 seeds: 12 (0)
T1 leaves: 5 (0)
 
35S::cl-CFP-flag-MaWSD5 91 T2 seeds: 21 (0)
T1 leaves: 38 (0)
 
  1. (Confocal microscopy
  2. Western blot
  3. §Analysis of WE species and WE amounts by GC-FID and nanoESI-MS/MS)