Fig. 2
From: Synthetic biology toolkit for engineering Cupriviadus necator H16 as a platform for CO2 valorization
Genome editing tools developed in C. necator H16. a Target gene deletion via group II introns. b Target gene deletion via two rounds of single-crossover using kanR and sacB as selection and counter-selection markers, respectively. c Target gene deletion via double-crossover using kanR as a selection marker and maker recycling by the Cre/loxP system. d Target gene deletion via CRISPR/Cas9. DSB: double-strand break