Table 1 Genetic engineering vectors commonly used in C. necator

Episomal plasmids

pCM

RP4 ORI, low copy, ~ 8

[47]

pCM271

RP4 ORI, mutation in trfA, medium copy, ~ 40

[47]

pBBR1

medium copy, ~ 40

[57]

pKT230

RSF1010 ORI, medium copy, ~ 10

[47]

pSa

low copy, 2 ~ 3

[46]

pCUP3

pMOL28 ORI, low copy, 1

[48]

Integrative plasmids

pJQPPCm

phaP (Phasin) as integration locus

[53, 54]

pJV7

phaC1 (PHA synthase) as integration locus

[51]

pCB42

phaB1 (acetoacetyl-CoA reductase) as integration locus

[66]

pLH63

phaB2C2 as integration locus

[26]

pEX100T

ldh (L-lactate dehydrogenase) as integration locus

[52]

pLO1

norR2A2B2 (NO reductase) as integration locus

[49, 68]