Table 2 Screening markers available in C. necator
From: Synthetic biology toolkit for engineering Cupriviadus necator H16 as a platform for CO2 valorization
Marker | Description | Reference |
|---|---|---|
Resistance marker | ||
NeoR/KanR | Resistant to kanamycin; 200–350 μg/mL | [47] |
TcR | Resistant to tetracycline; 10–25 μg/mL | [47] |
CmR | Resistant to chloramphenicol; 25–50 μg/mL | [47] |
Nutrition-deficient complementary marker | ||
hoxABCJ | C. necatorΔhoxA; transcription regulator of two [NiFe]-Hydrogenases | [49] |
eda | C. necatorΔeda; key enzyme of ED pathway | [52] |
xfp | C. necatorΔeda; key enzyme of the alternative phosphoketolase-dependent pathway | [50] |
proC | C. necator ΔproC; indispensable gene for proline biosynthesis | [51] |
Counter-selectable marker | ||
sacB | Lethal in sucrose-containing medium | [114] |