Table 3 Comparison of inducible promoters developed in C. necator

AraC/P_araBAD

10 mM L-arabinose

1200

Inhibited cell growth; not consumed

RhaRS/P_rhaBAD

11 mM L-rhamnose

1960

Virtually not altered cell growth; not consumed

LacI/P_lac

1 mM IPTG

 ~ 300

Inhibited cell growth at high concentration; not consumed

PM/P_xyls

1 mM m-toluic acid

 ~ 300

–

AcuR/P_acuRI

5 mM acrylate

33

Consumed

YpItcR/P_ccl

5 mM itaconate

105

Not consumed

MmsR/P_mmsA

10 mM 3-hydroxypropionic acid

51.5

Growth-retarding effect at the beginning of cultivation; consumed

HpdR/P_hpdH

10 mM 3-hydroxypropionic acid

516.6

Growth-retarding effect at the beginning of cultivation; consumed

LacI/P_j5-lac

1 mM IPTG

 ~ 7

Growth inhibition at high concentration; not consumed

CymR/P_j5-cmt

120 μM p-cumate

22

Nearly no effect on growth

TetR/P_rrsC-tetO

200 ng/mL anhydrotetracycline

 ~ 1100

No negative effect on growth; not consumed; antibiotic

TetR/P_tolC-tetO

25 ng/mL doxycycline

 ~ 180

No negative effect on growth; not consumed; antibiotic