Table 1 Plasmids and strains used in this work

Plasmids

 pCOM_AlkL

pCOM10 vector containing AlkL gene; Km^R

[22]

 pBTL10

pCOM10 vector containing AlkBGTL genes; Km^R

[50]

 pBGTL-Atf1

pCOM10 vector containing AlkBGTL-Atf1 genes; Km^R

[19]

 pSEVAb628

Expression vector; oriV(RK2); XylS, Pm; Gm^R

[47]

 pSEVAb658

Expression vector; oriV(RSF1010); XylS, Pm; Gm^R

[47]

 pSEVAb628-AlkS

pSEVAb628 vector containing Pm → AlkS; Gm^R

This work

 pSEVAb658-B^*G^*TLA^*

pSEVAb658 vector containing Pm → AlkB^*G^*TL-Atf1^*; Gm^R

This work

 pET26b-EstX

pET26b vector containing T7 → EstX, X represents each putative esterase gene; Km^R

This work

 pGNW2

Suicide vector used for gene deletion in P. putida KT2440; oriV(R6K) containing P14g → msfGFP; Km^R

[43]

 pGNW2-ΔCrc

Derivative of vector pGNW2 containing HRs to delete Crc (PP_5292)

This work

 pGNW2-ΔCyoB

Derivative of vector pGNW2 containing HRs to delete CyoB (PP_0813)

This work

 pGNW2-ΔEstX

Derivative of vector pGNW2 containing HRs to delete EsteraseX (Table 2)

This work

 pGNW2-PP_2047-48

Derivative of vector pGNW2 containing HRs to delete PP_2047-48

This work

 pGNW2-PP_2136-37

Derivative of vector pGNW2 containing HRs to delete PP_2136-37

This work

 pGNW2-PP_2214-18

Derivative of vector pGNW2 containing HRs to delete PP_2214-18

This work

Strains

 P. putida KT2440

Wild-type strain, derived from P. putida mt-2[51]

[52]

 ΔCrc

P. putida KT2440ΔCrc

This work

 ΔCyoB

P. putida KT2440ΔCyoB

This work

 ΔCrcΔCyoB

P. putida KT2440ΔCrcΔCyoB

This work

 ΔEstBCPZ

P. putida KT2440ΔEstBCPZ

This work

 ΔEst12/K

P. putida KT2440ΔEst12/K

This work

 ΔEstBCPZ12/K

P. putida KT2440ΔEstBCPZΔEst12/K

This work

 P. putida ΔBOX

P. putida KT2440ΔPP_2047-48ΔPP_2136-37ΔPP_2214-18

This work

 P-L

P. putida KT2440 harboring pCOM_alkL

This work

 P-BGTL

P. putida KT2440 harboring pBTL10

This work

 P-BGTLA

P. putida KT2440 harboring pBGTL-Atf1

This work

 ΔCrc-BGTL

P. putida KT2440ΔCrc harboring pBTL10

This work

 ΔCyoB-BGTL

P. putida KT2440ΔCyoB harboring pBTL10

This work

 ΔCrcΔCyoB- BGTL

P. putida KT2440ΔCrcΔCyoB harboring pBTL10

This work

 ΔCrc-BGTLA

P. putida KT2440ΔCrc harboring pBGTL-Atf1

This work

 ΔCyoB-BGTLA

P. putida KT2440ΔCyoB harboring pBGTL-Atf1

This work

 ΔCrc-BGTLAS

P. putida KT2440ΔCrc harboring pBGTL-Atf1, and pSEVAb628-alkS

This work

 ΔCyoB-BGTLAS

P. putida KT2440ΔCyoB harboring pBGTL-Atf1, and pSEVAb628-alkS

This work

 P-B^*G^*TLA^*

P. putida KT2440 harboring pSEVAb658-B^*G^*TLA^*

This work

 ΔEst12/K- B^*G^*TLA^*

P. putida KT2440ΔEst12/K harboring pSEVAb658-B^*G^*TLA^*

This work

 E. coli blank

E. coli NEBT7 harboring pET26b

This work

 E. coli-EstX

E. coli NEBT7 harboring pET26b-EstX

This work