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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Overexpression of the rice BAHD acyltransferase AT10 increases xylan-bound p-coumarate and reduces lignin in Sorghum bicolor

Fig. 2

Expression of OsAT10 in sorghum. Schematic diagram of the construct used for sorghum transformation (a) and relative OsAT10 expression levels among eight independent transgenic lines (b). The T-DNA contains a selection marker for kanamycin resistance (nptII) under the control of a duplicated 35S promoter (p2 × 35S) from cauliflower mosaic virus (CaMV) and harbors the OsAT10opt gene sequence under the control of the promoter of a sorghum polyubiquitin gene (pSbUbi). T35S and TNOS denote the terminators from the CaMV 35S and Agrobacterium tumefaciens nopaline synthase genes, respectively. OsAT10 transcripts were detected using RT-qPCR. OsAT10 expression levels relative to that of the PP2A gene are shown. cDNA obtained from wild-type segregants were used as negative control. Values are means ± SD of four biological replicates (n = 4). nd, not detected

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