Fig. 2From: Overexpression of the rice BAHD acyltransferase AT10 increases xylan-bound p-coumarate and reduces lignin in Sorghum bicolorExpression of OsAT10 in sorghum. Schematic diagram of the construct used for sorghum transformation (a) and relative OsAT10 expression levels among eight independent transgenic lines (b). The T-DNA contains a selection marker for kanamycin resistance (nptII) under the control of a duplicated 35S promoter (p2 × 35S) from cauliflower mosaic virus (CaMV) and harbors the OsAT10opt gene sequence under the control of the promoter of a sorghum polyubiquitin gene (pSbUbi). T35S and TNOS denote the terminators from the CaMV 35S and Agrobacterium tumefaciens nopaline synthase genes, respectively. OsAT10 transcripts were detected using RT-qPCR. OsAT10 expression levels relative to that of the PP2A gene are shown. cDNA obtained from wild-type segregants were used as negative control. Values are means ± SD of four biological replicates (n = 4). nd, not detectedBack to article page