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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Salt stress improves thermotolerance and high-temperature bioethanol production of multi-stress-tolerant Pichia kudriavzevii by stimulating intracellular metabolism and inhibiting oxidative damage

Fig. 4

Effect of salt stress on the heat-induced oxidative stress in P. kudriavzevii. Effect of salt stress on the A ROS-positive cell rate and B dead cell rate of P. kudriavzevii incubated at 45 °C. C Representative flow cytometry dot plots after double staining with DCFH-DA and PI. Q1: DCF/PI+, ROS-negative cells/dead cells; Q2: DCF+/PI+, ROS-positive cells/dead cells; Q3: DCF/PI, ROS-negative cells/living cells; Q4: DCF+/PI, ROS-positive cells/living cells. Effects of salt stress on the D oxidative damage and E antioxidant enzyme activities of P. kudriavzevii incubated at 45 °C. Data labelled with different letters are statistically different at p < 0.05 applying one-way ANOVA and the Tukey test

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