Fig. 1

Scheme of mutation procedure (a), phenotypic investigation of T. afroharzianum and its mutants, grown on selection medium b and their cellulase activities in fermentation medium (c). TA is a wild type of T. afroharzianum; M-84 is the best mutant in first round of mutagenesis; ME-10 is the best mutant in second round of mutagenesis; MEA-12 is the best mutant in third round of mutagenesis. Error bars indicate the mean ± SD of three biological replicates and statistical significance was calculated by Student’s t test (* (p < 0.05) and ** (p < 0.01))