Cellulase production and efficient saccharification of biomass by a new mutant Trichoderma afroharzianum MEA-12

Table 1 Number and positive mutation rate of wild strain T. afroharzianum obtained by three rounds of mutagenesis

Mutagenic round	Mutagenic method	Total number of mutants	Number of strains screened by enzyme activity				Positive mutation rate (%)
Mutagenic round	Mutagenic method	Total number of mutants	−	+	++	+++	Positive mutation rate (%)
Round 1	MNNG	84	60	19	4	1	28.57
Round 2	EMS	102	75	17	6	4	26.47
Round 3	ARTP	86	61	11	8	6	29.07

“−” refers to the negative mutants; “+” refers to the mutants with low activity; “++” refers to the mutants with middle activity; “+++” refers to the mutants with high activity

ISSN: 2731-3654