Fig. 5

Comparison of XI variants. a SDS-PAGE analysis of the culture supernatant of xylose-assimilating strains expressing four kinds of XIs. Each strain was cultured in YPX medium containing 2% xylose, and 0.6 mL of culture supernatant at the indicated times was analysed after precipitation. b Confirmation of optimal pH of the mutant XIs. c Comparison of the relative activity of the purified wild type XI and XI-76. The xylA protein band is indicated by an arrow. Mean values and standard deviations of triplicates are shown