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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Synergistic effects of multiple enzymes from industrial Aspergillus niger strain O1 on starch saccharification

Fig. 2

The processes of isolation and identification of starch saccharification-related enzymes from secreted proteins of A. niger O1. a (up) SDS-PAGE of the supernatants of the industrial glucoamylase-producing A. niger O1 after 6 days cultivation. (down) Isolation and purification of enzymes related to starch saccharification from secreted proteins of O1 using anion exchange chromatography. b (up) SDS-PAGE of isolated enzymes related to starch saccharification from secreted proteins of O1. Four lanes and M respectively represent O1-1, O1-2, O1-3, O1-4 and protein ladder. (down) LC-MS/MS detection of isolated proteins. O1-1 is neutral α-amylase, O1-3 is acid α-amylase, and O1-2 and O1-4 are glucoamylases with different glycosylation level

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