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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Construction of a tunable promoter library to optimize gene expression in Methylomonas sp. DH-1, a methanotroph, and its application to cadaverine production

Fig. 1

Construction of a library of promoter candidates in Methylomonas sp. DH-1. A In silico approach to predict promoters from the genomic sequences of Methylomonas sp. DH-1 and M. trichosporium OB3b and the functional classification of the coding sequences under the control of the predicted promoters. B 2D-PAGE approach to identify strong native promoters that allow for the overexpression of Methylomonas sp. DH-1. C Addition of four well-known promoters of M. trichosporium OB3b and Escherichia coli to the library

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