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Table 1 Tunable promoter library for Methylomonas sp. DH-1

From: Construction of a tunable promoter library to optimize gene expression in Methylomonas sp. DH-1, a methanotroph, and its application to cadaverine production

Promoter identification method Gene name Promoter strength (%)
In silico prediction (Methylomonas sp. DH-1) 50S ribosomal protein L28 (rpmB) 22.09
Fur family transcriptional regulator 14.99
50S ribosomal protein L21 (rplU) 7.16
Shikimate kinase (aroK) 6.49
SsrA-binding protein (smpB) 3.79
Phosphate ABC transporter permease 2.94
TetR family transcriptional regulator 2.7
ABC transporter 2.21
LysR family transcriptional regulator 1.68
16S ribosomal RNA 0.54
Glycosyl transferase 0.39
Cytochrome c oxidase subunit 2 0.0
Elongation factor P (efp) 0.0
50S ribosomal protein L31 (rpmE) 0.0
10 kDa chaperonin (groS) 0.0
In silico prediction (M. trichosporium OB3b) ATP-binding protein 191.08
(2Fe–2S)-binding protein 41.54
Ribosomal RNA small methyltransferase I (rsmI) 28.42
Peptidase S8 12.92
Iron–sulphur cluster assembly scaffold protein 7.45
Integrase 3.45
tRNA-dihydrouridine(20/20a) synthase (dusA) 2.91
Chromosomal replication initiator protein DnaA 2.81
Cytochrome c peroxidase 1.84
Thioredoxin peroxiredoxin 1.71
dTDP-glucose 4,6-dehydratase (rfbB) 0.56
Restriction endonuclease 0.43
Carbonic anhydrase 0.24
GGDEF domain-containing protein 0.0
2D-PAGE (Methylomonas sp. DH-1) Methanol dehydrogenase (mxaF) 411.18
Transaldolase (tal) 262.9
Glutamine synthetase (glnA) 68.74
3-hexulose-6-phosphate synthase (hps) 67.37
Elongation factor Tu (tuf) 16.12
M. trichosporium OB3b promoters Methane monooxygenase (pmoC) promoter C 1.58
Methanol dehydrogenase (mxaF) promoter 0.51
E. coli promoters lac promoter (without operator) 100
tac promoter (without operator) 20.54