Fig. 1

Cell growth of strain ZMP-PiXI (a), ZMP-PiXI-xylB (b), ZMP-RsXI (c), ZMP-RsXI-xylB (d), ZMP-RuXI (e) and ZMP-RuXI-xylB (f) in RMX5. Strains were cultured in 300 μL RMX5 with 50 μg/mL spectinomycin in 500 μL microporous honeycomb panel by Bioscreen C instrument at 30 °C. The induced concentration of tetracycline is 0 and 0.8 μg/mL, the plate was shaken before measuring the OD_{600 nm} every 3 min. Three replicates were performed for each experiment. The diagram in each panel represented plasmid constructs expressing XI alone or both XI and XK genes controlled by Ptet promoter (pink triangle) and rrnB T1 terminator (grey triangle). PiXI, RsXI, and RuXI represented genes encoding xylose isomerase (XI) from Piromyces sp. E2, protists in the Reticulitermes speratus hindgut, and bovine rumen contents, respectively; xylB was xylulokinase gene from E. coli. Plasmid containing the reporter gene EGFP was used as the control