Fig. 3From: Co‑cultivation of the anaerobic fungus Caecomyces churrovis with Methanobacterium bryantii enhances transcription of carbohydrate binding modules, dockerins, and pyruvate formate lyases on specific substratesA heatmap of the proportion of genes containing domains belonging to CAZyme gene families or associated enzymatic machinery upregulated in co-cultures of three different fungal strains paired with the same non-native methanogen, Methanobacterium bryantii relative to fungal monocultures grown on a reed canary grass substrate. Three different strains of anaerobic fungi, Anaeromyces robustus, Neocallimastix californiae, and Caecomyces churrovis were used to form separate co-cultures with M. bryantii and grown on a reed canary grass substrate along with monocultures of each fungus on the same substrate. Differential expression of fungal genes in co-cultures relative to fungal monocultures was determined using DESEQ2. Gene domains were organized into the following classifications: carbohydrate binding modules (CBM), dockerins (DOC), glycoside hydrolases (GH), and glycosyltransferases (GT), polysaccharide lyases (PL), and carbohydrate esterases (CE)Back to article page