Fig. 5

Effects of overexpressing ESRTs on secretory expressions of lignocellulolytic enzymes. Effects of overexpressing ESRTs on secretory expressions of AnFaeA (a) and RuCelA (b). AnFaeA or RuCelA was integrated at INU1 loci in the genome. Overexpression of the ESRT was driven by a TEF promoter on a multi-copy plasmid. The activity of AnFaeA or RuCelA in the supernatant of cells transformed with a void vector was designated as unit 1. The column corresponding to the void vector control is in red. Value represented mean ± SD (n = 3) (*P < 0.05, **P < 0.01, ***P < 0.001). ESRTs that significantly improved and reduced extracellular activities are in red and blue, respectively. c Representative ESRTs that displayed significant effects on secretory expressions of lignocellulolytic enzymes (*P < 0.05, **P < 0.01, *** P < 0.001). d Effects of known UPR target genes on secretory expressions of lignocellulolytic enzymes