Fig. 4From: Improved methanol tolerance of Rhizomucor miehei lipase based on N‑glycosylation within the α-helix region and its application in biodiesel productionThermostability assay of ProRML. Thermostability of the enzyme was determined using PNPP as the substrate. The thermostability was measured by evaluating the residual activity after enzyme was pre-incubated for 1 h at different temperatures (A) and pre-incubated for 24 h at 45 °C (B). The activity of the unheated sample was set to 100%, and the relative hydrolytic activity at each time point is presented as a percentageBack to article page