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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Improved methanol tolerance of Rhizomucor miehei lipase based on N‑glycosylation within the α-helix region and its application in biodiesel production

Fig. 4

Thermostability assay of ProRML. Thermostability of the enzyme was determined using PNPP as the substrate. The thermostability was measured by evaluating the residual activity after enzyme was pre-incubated for 1 h at different temperatures (A) and pre-incubated for 24 h at 45 °C (B). The activity of the unheated sample was set to 100%, and the relative hydrolytic activity at each time point is presented as a percentage

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