Fig. 5

Methanol tolerance of ProRML. Methanol tolerance of the enzyme was determined using PNPP as the substrate. The methanol tolerance was measured by evaluating the residual activity after enzyme was pre-incubated for 1 h at different methanol concentrations (A) and pre-incubated for 8 h at a 50% methanol concentration (B). The activity of the unheated sample was set to 100%, and the relative hydrolytic activity at each time point is presented as a percentage