Improved methanol tolerance of Rhizomucor miehei lipase based on N‑glycosylation within the α-helix region and its application in biodiesel production

Table 1 Kinetic parameters and thermostability at 50 °C of WT, N9A/N59A, and glycosylation mutants

Mutants	Km (mM)	kcat (min⁻¹)	kcat/Km (min⁻¹ mM⁻¹)	Thermostability at 50 °C (2.5 h)
WT	1.16	105.29 ± 2.96	90.49 ± 2.55	26.92 ± 2.26
N9A/N59A	0.35	50.61 ± 2.31	145.35 ± 6.65	25.77 ± 9.83
N93	4.70	3816.33 ± 157.15	812.33 ± 33.45	12.79 ± 6.36
N115	0.64	435.51 ± 16.61	679.43 ± 25.91	8.62 ± 4.31
N183	0.36	71.15 ± 0.38	195.49 ± 1.05	14.66 ± 0.85
N260	1.25	293.27 ± 8.94	235.56 ± 7.18	10.47 ± 4.31
N218	13.2	51,322.99 ± 1563.66	3888.11 ± 118.46	29.02 ± 5.52

ISSN: 2731-3654