Skip to main content

Table 1 Kinetic parameters and thermostability at 50 °C of WT, N9A/N59A, and glycosylation mutants

From: Improved methanol tolerance of Rhizomucor miehei lipase based on N‑glycosylation within the α-helix region and its application in biodiesel production

Mutants Km (mM) kcat (min−1) kcat/Km (min−1 mM−1) Thermostability at 50 °C (2.5 h)
WT 1.16 105.29 ± 2.96 90.49 ± 2.55 26.92 ± 2.26
N9A/N59A 0.35 50.61 ± 2.31 145.35 ± 6.65 25.77 ± 9.83
N93 4.70 3816.33 ± 157.15 812.33 ± 33.45 12.79 ± 6.36
N115 0.64 435.51 ± 16.61 679.43 ± 25.91 8.62 ± 4.31
N183 0.36 71.15 ± 0.38 195.49 ± 1.05 14.66 ± 0.85
N260 1.25 293.27 ± 8.94 235.56 ± 7.18 10.47 ± 4.31
N218 13.2 51,322.99 ± 1563.66 3888.11 ± 118.46 29.02 ± 5.52