Table 1 Primer sequences for these CCM-related genes used in present real-time PCR analysis
| Primers | Gene ID | Sequence (5′-3′) | Annealing temperature (°C) | Amplicon size (bp) |
|---|---|---|---|---|
| rps | 7197743 |
Sense: CGAAGTCAACCAGGAAACCAA Antisense: GTGCAAGAGACCGGACATACC | 60 | 166 |
| tbp | 7204339 |
Sense: ACCGGAGTCAAGAGCACACAC Antisense: CGGAATGCGCGTATACCAGT | 60 | 175 |
| ica | 7196274 |
Sense: GGGAACTGAGGCTGGAAC Antisense: CGAGCATGGAGACGGTAG | 60 | 240 |
| pepck | 7195549 |
Sense: AGCGGAACTGGAAAGACC Antisense: CATCGTCCCGAATAGCC | 60 | 208 |
| ppdk | 7203153 |
Sense: CGCCAAGGCAACAGGTAA Antisense: GGTGCTCAGCCGTCAAAT | 60 | 170 |
| pepc 1 | 7201832 |
Sense: TGACAAGAGTGCCTCGGGTATT Antisense: CAGCCACTTCGTTTCCTGTTTC | 60 | 134 |
| pepc 2 | 7203602 |
Sense: AACCCATCCGTCTATCGT Antisense: GGTACTCGGCACAACTCAC | 60 | 241 |
| NADP-me | 7201046 |
Sense: CATTGGGCTTGGCGTATT Antisense: TGCTACTCCCGTTTCGTG | 60 | 202 |
| NAD-me1 | 7197107 |
Sense: ATTGCTGGTGCGGGTAGT Antisense: TTGTAACGCCGAGAAGGA | 60 | 250 |
| mdh | 7199385 |
Sense: CGCTTCCGTGGCTTCTTA Antisense: CCTGGTCGGGATGTGACTG | 60 | 208 |
| pyc1 | 7198165 |
Sense: TTGGCTTTGTCCGTCAG Antisense: TGTTCCAGCAGTATCGTGT | 60 | 164 |
| pyc2 | 7195500 |
Sense: CGTCCGACTCACATCACAA Antisense: GAACCAAACCAACTTCCTCC | 60 | 206 |