Fig. 1

The results of TAP-MS and BiFC. Western blot (A) and SDS-PAGE with silver staining (B) of T. reesei QP4 (control) and TrCRE1-TAP strains. The green arrow, blue arrow, and red arrow represent proteins TrCYC8, TrTUP1, and TrCRE1, respectively. Western blot (C) and SDS-PAGE with silver staining (D) of P. oxalicum 114-2 (control) and PoCreA-TAP strains. Western blot (E) and SDS-PAGE with silver staining (F) of P. oxalicum 114-2 (control) and PoCyc8-TAP strains. The orange arrow and gray arrow represent proteins PoCyc8 and PoTup1, respectively. Western blot was performed using the ANTI-HA antibody (ABclonal, China). G Microscopy of PoCyc8-YFP-PoCreA BiFC strain; H Microscopy of PoTup1-YFP-PoCreA BiFC strain. Each image includes four parts. The upper left, blue particles indicate nucleus stained with Hoechst 33342. The upper right, normal white light. The bottom right, yellow fluorescent particles indicate interactions between two target proteins. The bottom left, indicating the merge of yellow fluorescence and blue nucleus