Fig. 3

Strategy and results of ChIP-qPCR. A Microscopy of PoCyc8-YFP-PoSet2 BiFC strain. The image includes four parts. The upper left, blue particles indicate nucleus stained with Hoechst 33342. The upper right, normal white light. The bottom right, yellow fluorescent particles indicate interactions between two PoCyc8 and PoSet2. The bottom left, indicating the merge of yellow fluorescence and blue nucleus. B Overview on the upstream sequence and core promoters of Pochb1 and Poegl1. The transcription start site (TSS) is designated as + 1. The initiator (Inr) and TATA box were illustrated. The three chromatin regions investigated by ChIP-qPCR are indicated by green, red, and blue bars, respectively. For Pochb1, region 1 covers from − 439 to − 263; region 2 covers from − 232 to − 51; region 3 covers from − 73 to + 95. For Poegl1, region 1 covers from − 512 to − 344; region 2 covers from − 316 to − 142; region 3 covers from − 155 to + 30. The putative DNA-binding sites of PoCreA (5′-GCGGAG-3′; 5′-CCGGGG-3′; 5′-CCCCGC-3′; 5′-CCCCGG-3′; 5′-CTCCGG-3′) are indicated by orange triangles. The orientation of the triangle represents the orientation of the binding motif. Inr, initiator element; TATA, TATA box. C ChIP-qPCR for H3K36me2 of Pocbh1. D ChIP-qPCR for H3K36me2 of Poegl1. All values are means from measurements in triplicates and three biological experiments. The error bars indicate standard deviations. *P < 0.05, **P < 0.01, ***P < 0.001