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Table 1 The top 10 proteins interacting with TrCRE1 identified through TAP-MS

From: Carbon catabolite repression involves physical interaction of the transcription factor CRE1/CreA and the Tup1–Cyc8 complex in Penicillium oxalicum and Trichoderma reesei

Rank Protein ID emPAIa T. reesei QM6a S. cerevisiae S288C Predicted function
Protein Homologue Identity % E value Locationb
1st Trire2_120117 6.58 × 1025 TrCRE1 Mig1 72 7e−30 Nucleus and cytoplasm Sequence-specific DNA binding transcription factor involved in the regulation of transcription by RNA polymerase II in response to glucose and starvation
2nd Trire2_120053 1.09 × 105 TrHSP70 Ssc1 74 0.0 Mitochondrion Hsp70 family ATPase, involving in protein importing and folding
3rd Trire2_121940 1.29 × 103 TrTUP1 Tup1 49 2e−128 Nucleus General repressor of transcription, forms complex with Cyc8p
4th Trire2_102616 3.82 × 102 TrCYC8 Cyc8 55 1e−136 Nucleus General repressor of transcription, forms complex with Tup1p
5th Trire2_122572 1.18 × 102 Ssb2 73 0.0 Cytoplasm and membrane Cytoplasmic ATPase that is a ribosome-associated molecular chaperone
6th Trire2_122920 6.31 TrBIP1 Kar2 76 0.0 Endoplasmic reticulum ATPase involved in protein import into the endoplasmic reticulum
7th Trire2_121906 4.01 TrRPS14 Rps14A 84 6e−63 Nucleus and cytoplasm mRNA-binding constituent of the cytosolic small ribosomal subunit; involved in maturation of the small subunit rRNA and assembly of the small ribosomal subunit
8th Trire2_119855 3.96 TrRPS3 Rps3 75 3e−124 Cytoplasm 40 s ribosomal protein
9th Trire2_102864 3.64 Uncharacterized protein
10th Trire2_123753 2.98 TrRPS31 Rps31 85 8e−67 Nucleus and cytoplasm Fusion protein that is cleaved to yield ubiquitin and a subunit of the cytosolic small ribosomal subunit; involved in maturation of the small subunit rRNA, assembly of the small ribosomal subunit, and translation
  1. –: No significant similarity found
  2. aemPAI is the Exponentially Modified Protein Abundance Index of three samples. Every Peptide count of each sample is listed in Additional file 3: Spreadsheet S1
  3. bData from Saccharomyces Genome Database (www.yeastgenome.org)