Table 1 The top 10 proteins interacting with TrCRE1 identified through TAP-MS
Rank | Protein ID | emPAIa | T. reesei QM6a | S. cerevisiae S288C | Predicted function | |||
|---|---|---|---|---|---|---|---|---|
Protein | Homologue | Identity % | E value | Locationb | ||||
1st | Trire2_120117 | 6.58 × 1025 | TrCRE1 | Mig1 | 72 | 7e−30 | Nucleus and cytoplasm | Sequence-specific DNA binding transcription factor involved in the regulation of transcription by RNA polymerase II in response to glucose and starvation |
2nd | Trire2_120053 | 1.09 × 105 | TrHSP70 | Ssc1 | 74 | 0.0 | Mitochondrion | Hsp70 family ATPase, involving in protein importing and folding |
3rd | Trire2_121940 | 1.29 × 103 | TrTUP1 | Tup1 | 49 | 2e−128 | Nucleus | General repressor of transcription, forms complex with Cyc8p |
4th | Trire2_102616 | 3.82 × 102 | TrCYC8 | Cyc8 | 55 | 1e−136 | Nucleus | General repressor of transcription, forms complex with Tup1p |
5th | Trire2_122572 | 1.18 × 102 | – | Ssb2 | 73 | 0.0 | Cytoplasm and membrane | Cytoplasmic ATPase that is a ribosome-associated molecular chaperone |
6th | Trire2_122920 | 6.31 | TrBIP1 | Kar2 | 76 | 0.0 | Endoplasmic reticulum | ATPase involved in protein import into the endoplasmic reticulum |
7th | Trire2_121906 | 4.01 | TrRPS14 | Rps14A | 84 | 6e−63 | Nucleus and cytoplasm | mRNA-binding constituent of the cytosolic small ribosomal subunit; involved in maturation of the small subunit rRNA and assembly of the small ribosomal subunit |
8th | Trire2_119855 | 3.96 | TrRPS3 | Rps3 | 75 | 3e−124 | Cytoplasm | 40 s ribosomal protein |
9th | Trire2_102864 | 3.64 | – | – | – | – | – | Uncharacterized protein |
10th | Trire2_123753 | 2.98 | TrRPS31 | Rps31 | 85 | 8e−67 | Nucleus and cytoplasm | Fusion protein that is cleaved to yield ubiquitin and a subunit of the cytosolic small ribosomal subunit; involved in maturation of the small subunit rRNA, assembly of the small ribosomal subunit, and translation |