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Table 2 The top 10 proteins interacting with PoCreA identified through TAP-MS

From: Carbon catabolite repression involves physical interaction of the transcription factor CRE1/CreA and the Tup1–Cyc8 complex in Penicillium oxalicum and Trichoderma reesei

Rank Gene locus emPAIa P. oxalicum 114-2 S. cerevisiae S288C Predicted function
Protein Homologue Identity % E value Locationb
1st PDE_03168 2.01 × 102 PoCreA Mig1 68 2e−29 Nucleus and cytoplasm Sequence-specific DNA binding transcription factor involved in the regulation of transcription by RNA polymerase II in response to glucose and starvation
2nd PDE_01024 11.18 PoTup1 Tup1 48 2e−128 Nucleus General repressor of transcription, forms complex with Cyc8p
3rd PDE_03177 2.16 PoCyc8 Cyc8 58 7e−149 Nucleus General repressor of transcription, forms complex with Tup1p
4th PDE_04157 1.22 Initiation-specific alpha-1,6-mannosyltransferase
5th PDE_09900 0.74 Thi13 66 3e−175 Unknown Protein involved in synthesis of the thiamine precursor HMP
6th PDE_02746 0.50 Putative protein
7th PDE_09681 0.49 Sps19 49 2e−87 Peroxisome Peroxisomal 2,4-dienoyl-CoA reductase involved in fatty acid catabolism and sporulation
8th PDE_07279 0.47 Atp2 79 0.0 Mitochondrion Subunit of the catalytic core of the F1 sector of mitochondrial F1F0 ATP synthase
9th PDE_04469 0.33 Cct2 74 0.0 Cytoplasm Subunit of the chaperonin-containing T-complex (TriC) that mediates protein folding
10th PDE_03408 0.32 Cdc19 66 0.0 Cytoplasm Pyruvate kinase that catalyzes the final step in glycolysis, the conversion of phosphoenolpyruvate to pyruvate, which is then utilized in anaerobic or aerobic respiration
  1. –: No significant similarity found
  2. aemPAI is the Exponentially Modified Protein Abundance Index of three samples. Every Peptide count of each sample is listed in Additional file 3: Spreadsheet S1
  3. bData from Saccharomyces Genome Database (www.yeastgenome.org)