Table 2 The top 10 proteins interacting with PoCreA identified through TAP-MS

1st

PDE_03168

2.01 × 10²

PoCreA

Mig1

68

2e−29

Nucleus and cytoplasm

Sequence-specific DNA binding transcription factor involved in the regulation of transcription by RNA polymerase II in response to glucose and starvation

2nd

PDE_01024

11.18

PoTup1

Tup1

48

2e−128

Nucleus

General repressor of transcription, forms complex with Cyc8p

3rd

PDE_03177

2.16

PoCyc8

Cyc8

58

7e−149

Nucleus

General repressor of transcription, forms complex with Tup1p

4th

PDE_04157

1.22

–

–

–

–

–

Initiation-specific alpha-1,6-mannosyltransferase

5th

PDE_09900

0.74

–

Thi13

66

3e−175

Unknown

Protein involved in synthesis of the thiamine precursor HMP

6th

PDE_02746

0.50

–

–

–

–

–

Putative protein

7th

PDE_09681

0.49

–

Sps19

49

2e−87

Peroxisome

Peroxisomal 2,4-dienoyl-CoA reductase involved in fatty acid catabolism and sporulation

8th

PDE_07279

0.47

–

Atp2

79

0.0

Mitochondrion

Subunit of the catalytic core of the F1 sector of mitochondrial F1F0 ATP synthase

9th

PDE_04469

0.33

–

Cct2

74

0.0

Cytoplasm

Subunit of the chaperonin-containing T-complex (TriC) that mediates protein folding

10th

PDE_03408

0.32

–

Cdc19

66

0.0

Cytoplasm

Pyruvate kinase that catalyzes the final step in glycolysis, the conversion of phosphoenolpyruvate to pyruvate, which is then utilized in anaerobic or aerobic respiration