Carbon catabolite repression involves physical interaction of the transcription factor CRE1/CreA and the Tup1–Cyc8 complex in Penicillium oxalicum and Trichoderma reesei

Table 2 The top 10 proteins interacting with PoCreA identified through TAP-MS

Rank	Gene locus	emPAI^a	P. oxalicum 114-2	S. cerevisiae S288C				Predicted function
Rank	Gene locus	emPAI^a	Protein	Homologue	Identity %	E value	Location^b	Predicted function
1st	PDE_03168	2.01 × 10²	PoCreA	Mig1	68	2e−29	Nucleus and cytoplasm	Sequence-specific DNA binding transcription factor involved in the regulation of transcription by RNA polymerase II in response to glucose and starvation
2nd	PDE_01024	11.18	PoTup1	Tup1	48	2e−128	Nucleus	General repressor of transcription, forms complex with Cyc8p
3rd	PDE_03177	2.16	PoCyc8	Cyc8	58	7e−149	Nucleus	General repressor of transcription, forms complex with Tup1p
4th	PDE_04157	1.22	–	–	–	–	–	Initiation-specific alpha-1,6-mannosyltransferase
5th	PDE_09900	0.74	–	Thi13	66	3e−175	Unknown	Protein involved in synthesis of the thiamine precursor HMP
6th	PDE_02746	0.50	–	–	–	–	–	Putative protein
7th	PDE_09681	0.49	–	Sps19	49	2e−87	Peroxisome	Peroxisomal 2,4-dienoyl-CoA reductase involved in fatty acid catabolism and sporulation
8th	PDE_07279	0.47	–	Atp2	79	0.0	Mitochondrion	Subunit of the catalytic core of the F1 sector of mitochondrial F1F0 ATP synthase
9th	PDE_04469	0.33	–	Cct2	74	0.0	Cytoplasm	Subunit of the chaperonin-containing T-complex (TriC) that mediates protein folding
10th	PDE_03408	0.32	–	Cdc19	66	0.0	Cytoplasm	Pyruvate kinase that catalyzes the final step in glycolysis, the conversion of phosphoenolpyruvate to pyruvate, which is then utilized in anaerobic or aerobic respiration

–: No significant similarity found
^aemPAI is the Exponentially Modified Protein Abundance Index of three samples. Every Peptide count of each sample is listed in Additional file 3: Spreadsheet S1
^bData from Saccharomyces Genome Database (www.yeastgenome.org)

ISSN: 2731-3654