Table 3 The top 10 proteins interacting with PoCyc8 identified through TAP-MS
Rank | Gene locus | emPAIa | P.oxalicum 114-2 | S. cerevisiae S288C | Predicted function | |||
|---|---|---|---|---|---|---|---|---|
Protein | Homologue | Identity % | E value | Locationb | ||||
1st | PDE_03177 | 2.63 × 1011 | PoCyc8 | Cyc8 | 58 | 7e−149 | Nucleus | General repressor of transcription, forms complex with Tup1p |
2nd | PDE_01024 | 1.39 × 108 | PoTup1 | Tup1 | 48 | 2e−128 | Nucleus | General repressor of transcription, forms complex with Cyc8p |
3rd | PDE_02075 | 3.16 × 103 | – | Nfs1 | 74 | 0.0 | Nucleus and mitochondrion | Mitochondrial cysteine desulfurase involved in iron-sulfur cluster assembly, tRNA thio-modification and tRNA wobble uridine modification; subunit of l-cysteine desulfurase complex |
4th | PDE_05635 | 1.92 × 102 | – | Rpl38 | 49 | 2e−18 | Cytoplasm and ribosome | Subunit of the cytosolic large ribosomal subunit; involved in translation |
5th | PDE_08686 | 18.14 | – | Vma2 | 85 | 0.0 | Cytoplasm and vacuole membrane | Hydrogen ion transporting ATPase involved in vacuolar acidification, calcium homeostasis, and the assembly of proteasome storage granules |
6th | PDE_05790 | 7.80 | – | Rpt6 | 77 | 0.0 | Nucleus and cytoplasm | Putative ATPase involved in proteasome regulatory particle assembly |
7th | PDE_01647 | 6.80 | – | Rpt1 | 77 | 0.0 | Nucleus and cytoplasm | Putative ATPase involved in proteasome regulatory particle assembly |
8th | PDE_00902 | 6.02 | – | Rpb11 | 50 | 4e−38 | Nucleus | DNA-directed RNA polymerase II subunit RPB11 |
9th | PDE_08458 | 5.45 | – | Psa1 | 73 | 0.0 | Nucleus and cytoplasm | Mannose-1-phosphate guanyltransferase; synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis |
10th | PDE_07279 | 5.39 | – | Atp2 | 79 | 0.0 | Mitochondrion | Subunit of the catalytic core of the F1 sector of mitochondrial F1F0 ATP synthase |
…… | …… | …… | …… | …… | …… | …… | …… | …… |
50th | PDE_03168 | 0.35 | PoCreA | Mig1 | 68 | 2e−29 | Nucleus and cytoplasm | Sequence-specific DNA binding transcription factor involved in the regulation of transcription by RNA polymerase II in response to glucose and starvation |