Skip to main content

Table 3 The top 10 proteins interacting with PoCyc8 identified through TAP-MS

From: Carbon catabolite repression involves physical interaction of the transcription factor CRE1/CreA and the Tup1–Cyc8 complex in Penicillium oxalicum and Trichoderma reesei

Rank Gene locus emPAIa P.oxalicum 114-2 S. cerevisiae S288C Predicted function
Protein Homologue Identity % E value Locationb
1st PDE_03177 2.63 × 1011 PoCyc8 Cyc8 58 7e−149 Nucleus General repressor of transcription, forms complex with Tup1p
2nd PDE_01024 1.39 × 108 PoTup1 Tup1 48 2e−128 Nucleus General repressor of transcription, forms complex with Cyc8p
3rd PDE_02075 3.16 × 103 Nfs1 74 0.0 Nucleus and mitochondrion Mitochondrial cysteine desulfurase involved in iron-sulfur cluster assembly, tRNA thio-modification and tRNA wobble uridine modification; subunit of l-cysteine desulfurase complex
4th PDE_05635 1.92 × 102 Rpl38 49 2e−18 Cytoplasm and ribosome Subunit of the cytosolic large ribosomal subunit; involved in translation
5th PDE_08686 18.14 Vma2 85 0.0 Cytoplasm and vacuole membrane Hydrogen ion transporting ATPase involved in vacuolar acidification, calcium homeostasis, and the assembly of proteasome storage granules
6th PDE_05790 7.80 Rpt6 77 0.0 Nucleus and cytoplasm Putative ATPase involved in proteasome regulatory particle assembly
7th PDE_01647 6.80 Rpt1 77 0.0 Nucleus and cytoplasm Putative ATPase involved in proteasome regulatory particle assembly
8th PDE_00902 6.02 Rpb11 50 4e−38 Nucleus DNA-directed RNA polymerase II subunit RPB11
9th PDE_08458 5.45 Psa1 73 0.0 Nucleus and cytoplasm Mannose-1-phosphate guanyltransferase; synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis
10th PDE_07279 5.39 Atp2 79 0.0 Mitochondrion Subunit of the catalytic core of the F1 sector of mitochondrial F1F0 ATP synthase
…… …… …… …… …… …… …… …… ……
50th PDE_03168 0.35 PoCreA Mig1 68 2e−29 Nucleus and cytoplasm Sequence-specific DNA binding transcription factor involved in the regulation of transcription by RNA polymerase II in response to glucose and starvation
  1. aemPAI is the Exponentially Modified Protein Abundance Index of three samples. Every Peptide count of each sample is listed in the Additional file 3: Spreadsheet S1
  2. bData from Saccharomyces Genome Database (www.yeastgenome.org)
  3. –: No significant similarity found