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Table 1 Strains and plasmids used in this study

From: Enhanced production of ectoine from methane using metabolically engineered Methylomicrobium alcaliphilum 20Z

  Characteristics References or source
Strains
 Escherichia coli DH10b   
 Escherichia coli S17-1 λpir Donor strain  
 Methylomicrobium alcaliphilum 20Z Used as host strain DSMZ
 M. alcaliphilum 20ZDP Mutant of M. alcaliphilum 20Z which was removed an endogenous plasmid by knocking out repB in endogenous plasmid locus This study
 M. alcaliphilum 20Z/dt M. alcaliphilum 20Z harboring pAWP89 This study
 M. alcaliphilum 20ZDP/dt M. alcaliphilum 20ZDP harboring pAWP89 This study
 M. alcaliphilum 20ZDP1 Mutant of M. alcaliphilum 20ZDP which was deleted an ectD This study
 M. alcaliphilum 20ZDP2 Mutant of M. alcaliphilum 20ZDP which was deleted an ectD and ectR This study
Vectors
 pAWP89 IncP-based broad host-range plasmid  
 pK19mobsacB Kanamycin resistant version of suicide vector pK19mobsacB This study
pK19repB pK19mobsacB containing flank regions of repB This study
pK19ectD pK19mobsacB containing flank regions of ectD This study
pK19ectDectR pK19mobsacB delete containing flank regions of ectR This study
M. alcaliphilum 20ZDP2 Mutant of M. alcaliphilum 20ZDP which was deleted an ectD and ectR This study