Table 1 Strains and plasmids used in this study
Characteristics | References or source | |
|---|---|---|
Strains | ||
Escherichia coli DH10b | ||
Escherichia coli S17-1 λpir | Donor strain | |
Methylomicrobium alcaliphilum 20Z | Used as host strain | DSMZ |
M. alcaliphilum 20ZDP | Mutant of M. alcaliphilum 20Z which was removed an endogenous plasmid by knocking out repB in endogenous plasmid locus | This study |
M. alcaliphilum 20Z/dt | M. alcaliphilum 20Z harboring pAWP89 | This study |
M. alcaliphilum 20ZDP/dt | M. alcaliphilum 20ZDP harboring pAWP89 | This study |
M. alcaliphilum 20ZDP1 | Mutant of M. alcaliphilum 20ZDP which was deleted an ectD | This study |
M. alcaliphilum 20ZDP2 | Mutant of M. alcaliphilum 20ZDP which was deleted an ectD and ectR | This study |
Vectors | ||
pAWP89 | IncP-based broad host-range plasmid | |
pK19mobsacB | Kanamycin resistant version of suicide vector pK19mobsacB | This study |
pK19â–³repB | pK19mobsacB containing flank regions of repB | This study |
pK19â–³ectD | pK19mobsacB containing flank regions of ectD | This study |
pK19â–³ectDâ–³ectR | pK19mobsacB delete containing flank regions of ectR | This study |
M. alcaliphilum 20ZDP2 | Mutant of M. alcaliphilum 20ZDP which was deleted an ectD and ectR | This study |