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Table 3 Oligonucleotides used in this study

From: Enhanced production of ectoine from methane using metabolically engineered Methylomicrobium alcaliphilum 20Z

Oligonucleotides

Sequence

repBDown F

TGACATGATTACGCCAAGCTTATGAAGGAATCGTTGTCGTACCC

repBDown R

ATATCGCCGCATTGGCAGGTTACGACCCTAAGC

repBUp F

ACCTGCCAATGCGGCGATATGGGGAAAAT

repBUp R

AAAACGACGGCCAGTGAATTCTTATGAAATGATCCGCCGTTTGTGC

repBDI F

TGTGTGGAATTGTGAGCGGA

repBDI R

GGTCGAGTGGCTTAAAACGC

repBUI F

GCCTTTCAACGGCCATCTTC

repBUI R

TTTCTGCGGACTGGCTTTCT

repB confirm F

TCCTTGGCCACCGAATTACC

repB confirm R

GCACTTTGCAACCCGACAAT

korB confirm F

ACCGTCTTGAGTTGGTCGTC

korB confirm R

AACTAAGAGCTTCGGCCCAC

trbF confirm F

CTAACCCGTACCTGACTGCC

trbF confirm R

ACGCACATAGATACCGAGCG

ectDDown F

TGACATGATTACGCCAAGCTTTGTTCAGCAACAAAAGACAGAA

ectDDown R

ATCACGTAACATGCTTAGAATGTTAATAATGTTGA

ectDUp F

TTCTAAGCATGTTACGTGATTGATAAAAATCTTCA

ectDUp R

AAAACGACGGCCAGTGAAAGTGAATTCTTGTACTGATGTTTCTTACCCCT

ectDDI F

CACTCATTAGGCACCCCAGG

ectDDI R

CAATGGAAAACCCGGCAGTG

ectDUI F

CACTGCCGGGTTTTCCATTG

ectDUI R

ACGACGGCCAGTGAATTCTT

ectD confirm F

TGCTTCATCGTTGACGCTCT

ectD confirm R

ATGGCAAGTCAGTCGAGCAA

ectR Down F

TGACATGATTACGCCAAGCTTTCTAATTCTCTCCTGAGCAAGATG

ectR Down R

TTATTAGAGCCATTGATCACCAATCATTAGAATAG

ectRUp F

GTGATCAATGGCTCTAATAATTCAGCTCAGCT

ectRUp R

AAAACGACGGCCAGTGAATTCAGAACATCAAGAGGTCTGGATTGT

ectRDI F

TTTGCTGGCCTTTTGCTCAC

ectRDI R

GTAAATCGGTGGCGCGAATC

ectRUI F

TACGTAGAGTGATTCGCGCC

ectRUI R

CGGACTGGCTTTCTACGTGT

ectR confirm F

GGGCATTTGCTAATCAGCCG

ectR confirm R

AACAGCACCGCTTCCAAGTA