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Fig. 5 | Biotechnology for Biofuels and Bioproducts

Fig. 5

From: Boosting the acetol production in methanotrophic biocatalyst Methylomonas sp. DH-1 by the coupling activity of heteroexpressed novel protein PmoD with endogenous particulate methane monooxygenase

Fig. 5

Proposed acetone assimilation pathway in Methylomonas sp. DH-1. A Organization of related genes in the proposed acetone assimilation pathway on the chromosome of the methanotrophic Methylomonas sp. DH-1. B Particulate methane monooxygenase (pMMO; AYM39_RS18590, AYM39_RS18595, and AYM39_RS18600) co-expressed with novel PmoD (IT6_09410) of Methylacidiphilum sp. IT6 oxidizes acetone to acetol, which could be further oxidized to methylglyoxal by GMC oxidoreductase (AYM39_RS20165). Passive diffusion or active transportation via transporter protein (ABC transporter) may be responsible for the methylglyoxal transportation into the cytoplasm. Seven candidate genes (AYM39_RS20250, AYM39_RS03110, AYM39_RS04650, AYM39_RS09045, AYM39_RS14435, AYM39_RS16860, AYM39_RS21550) annotated as glyoxalase enzymes may convert methylglyoxal into S-lactoylglutathione, which can be further converted into lactate by hydroxyacylglutathione hydrolase (AYM39_RS06120) and then to pyruvate by putative lactate dehydrogenase (AYM39_RS04895, AYM39_RS13600). The generated pyruvate can be either converted to acetyl-CoA to enter the TCA cycle or to phosphoenolpyruvate (PEP) by PEP-synthase (AYM39_RS13170). The related genes are shown using their locus tags obtained from the genome of Methylomonas sp. DH-1 (GenBank accession number NZ_CP014360). The related reactions of the proposed pathway are demonstrated by dashed arrows

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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