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Fig. 3 | Biotechnology for Biofuels and Bioproducts

Fig. 3

From: Activity-based protein profiling reveals dynamic substrate-specific cellulase secretion by saprotrophic basidiomycetes

Fig. 3

CAZymes identified in the pulldown from the day 10 secretomes using biotinylated ABP-Cel. Each plot shows a point for each protein detected (minimum 2 peptides at 1% FDR) in the day 10 secretome listed above the plot (AP = aspen pulp, WS = wheat straw). The x-axis is the number of spectra collected for peptides assigned to each protein (log2 scale) and the y-axis is the log2TMT127/126 ratio (127 = labelled, 126 = vehicle control) calculated by Scaffold for the protein, normalized using the TMT ratio of streptavidin. Points corresponding to putative retaining endo-β-glucanases/xylanases are coloured according to glycoside hydrolase family; other proteins are coloured dark grey. Detected contaminants not derived from the fungi under study (e.g. streptavidin, trypsin, keratins) are coloured light grey. A hyperbolic hit cut-off line is shown as a black dashed line with lower limits at 2 spectral counts and a 127/126 ratio of 4. Points found above this line are both well detected in the pulldown sample and depleted in the vehicle control. Source data (Excel format) can be found in Additional files 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 and 11. Plots were prepared using ggplot2

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