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Table 1 Comparisons of fermentation performance of engineered strains using original strain KF-7 as the host

From: Screening novel genes by a comprehensive strategy to construct multiple stress-tolerant industrial Saccharomyces cerevisiae with prominent bioethanol production

Indexes

Strains

8.0% (v/v)

Initial ethanol

(YPD150)

44 °C

(YPD100)

43 °C + 2.6% (v/v)

Initial ethanol

(YPD100)

27% Glucose

(YPD270)

1.25 M NaCl

(YPD150)

 

KF-7

18.79 ± 2.11c

31.39 ± 2.54c

13.00 ± 1.84b

123.92 ± 0.79c

43.78 ± 1.37d

Final generated ethanol concentration (g/L)

KF-7-CRZ1

14.82 ± 1.48d

40.11 ± 2.42ab

21.93 ± 1.49a

128.99 ± 1.05b

63.66 ± 1.50a

KF-7-ENA5

23.96 ± 0.73a

41.69 ± 0.66ab

22.50 ± 1.70a

131.65 ± 0.99a

64.26 ± 1.12a

KF-7ΔASP3

22.76 ± 1.12ab

44.37 ± 1.86a

25.91 ± 2.24a

128.19 ± 0.69b

45.16 ± 1.17d

 

KF-7ΔTOS8

20.26 ± 1.57ab

36.58 ± 2.19bc

14.63 ± 1.79b

129.40 ± 0.96ab

54.78 ± 0.81bc

 

KF-7ΔYOL162W

19.49 ± 0.94bc

17.70 ± 3.60d

4.24 ± 1.26c

122.80 ± 1.19c

57.77 ± 1.62b

 

KF-7ΔYOR012W

22.45 ± 1.11ab

37.63 ± 1.54bc

16.94 ± 1.43b

126.95 ± 1.99bc

51.16 ± 1.33c

 

KF-7

100.12 ± 4.07ab

29.51 ± 3.57b

71.80 ± 3.41b

25.09 ± 1.49a

40.18 ± 4.81a

Residual glucose (g/L)

KF-7-CRZ1

105.01 ± 3.12a

13.75 ± 3.78 cd

50.59 ± 2.80d

13.48 ± 2.58c

0.00 ± 0.00c

KF-7-ENA5

86.88 ± 1.55d

12.62 ± 4.69 cd

50.10 ± 3.37d

10.90 ± 1.88c

0.00 ± 0.00c

 

KF-7ΔASP3

89.53 ± 1.29 cd

5.89 ± 2.90d

41.76 ± 4.16d

15.05 ± 1.31bc

34.11 ± 2.72a

 

KF-7ΔTOS8

95.74 ± 2.71bc

19.88 ± 3.72bc

67.85 ± 3.33bc

13.74 ± 1.91c

9.76 ± 1.88b

 

KF-7ΔYOL162W

98.98 ± 1.86ab

57.75 ± 5.06a

85.36 ± 2.35a

29.66 ± 2.92a

1.36 ± 0.53c

 

KF-7ΔYOR012W

90.97 ± 1.13 cd

18.56 ± 2.63c

61.66 ± 2.84c

19.48 ± 1.17b

14.66 ± 3.09b

Ethanol yield (g ethanol/g consumed glucose)

KF-7

0.45 ± 0.01a

0.45 ± 0.01bc

0.47 ± 0.01a

0.50 ± 0.01a

0.42 ± 0.02ab

KF-7-CRZ1

0.41 ± 0.01b

0.46 ± 0.00ab

0.46 ± 0.01a

0.50 ± 0.00a

0.44 ± 0.02ab

KF-7-ENA5

0.44 ± 0.00a

0.48 ± 0.02a

0.46 ± 0.01a

0.50 ± 0.01a

0.45 ± 0.01a

 

KF-7ΔASP3

0.44 ± 0.00a

0.47 ± 0.00ab

0.46 ± 0.01a

0.50 ± 0.00a

0.41 ± 0.00bc

 

KF-7ΔTOS8

0.44 ± 0.02a

0.46 ± 0.01ab

0.47 ± 0.01a

0.49 ± 0.01a

0.41 ± 0.00bc

 

KF-7ΔYOL162W

0.46 ± 0.01a

0.42 ± 0.01c

0.32 ± 0.02b

0.49 ± 0.01a

0.41 ± 0.01bc

 

KF-7ΔYOR012W

0.45 ± 0.01a

0.46 ± 0.01ab

0.45 ± 0.02a

0.50 ± 0.01a

0.39 ± 0.01c

  1. The data in the table are those at the end of fermentation. Values indicate mean ± standard deviation of three biological replications. Values followed by different lowercase letters in the same column indicate significant differences at the level of P < 0.05 (Tukey-test) among strains. Same lowercase letters, no difference. KF-7-CRZ1: overexpression of TF Crz1p in KF-7; KF-7-ENA5: overexpression of ENA5 in KF-7; KF-7ΔASP3: Knockout ASP3 in KF-7; KF-7ΔTOS8: Knockout TOS8 in KF-7; KF-7ΔYOL162W: Knockout YOL162W in KF-7; KF-7ΔYOR012W: Knockout YOR012W in KF-7