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Table 1 Comparisons of fermentation performance of engineered strains using original strain KF-7 as the host

From: Screening novel genes by a comprehensive strategy to construct multiple stress-tolerant industrial Saccharomyces cerevisiae with prominent bioethanol production

Indexes Strains 8.0% (v/v)
Initial ethanol
(YPD150)
44 °C
(YPD100)
43 °C + 2.6% (v/v)
Initial ethanol
(YPD100)
27% Glucose
(YPD270)
1.25 M NaCl
(YPD150)
  KF-7 18.79 ± 2.11c 31.39 ± 2.54c 13.00 ± 1.84b 123.92 ± 0.79c 43.78 ± 1.37d
Final generated ethanol concentration (g/L) KF-7-CRZ1 14.82 ± 1.48d 40.11 ± 2.42ab 21.93 ± 1.49a 128.99 ± 1.05b 63.66 ± 1.50a
KF-7-ENA5 23.96 ± 0.73a 41.69 ± 0.66ab 22.50 ± 1.70a 131.65 ± 0.99a 64.26 ± 1.12a
KF-7ΔASP3 22.76 ± 1.12ab 44.37 ± 1.86a 25.91 ± 2.24a 128.19 ± 0.69b 45.16 ± 1.17d
  KF-7ΔTOS8 20.26 ± 1.57ab 36.58 ± 2.19bc 14.63 ± 1.79b 129.40 ± 0.96ab 54.78 ± 0.81bc
  KF-7ΔYOL162W 19.49 ± 0.94bc 17.70 ± 3.60d 4.24 ± 1.26c 122.80 ± 1.19c 57.77 ± 1.62b
  KF-7ΔYOR012W 22.45 ± 1.11ab 37.63 ± 1.54bc 16.94 ± 1.43b 126.95 ± 1.99bc 51.16 ± 1.33c
  KF-7 100.12 ± 4.07ab 29.51 ± 3.57b 71.80 ± 3.41b 25.09 ± 1.49a 40.18 ± 4.81a
Residual glucose (g/L) KF-7-CRZ1 105.01 ± 3.12a 13.75 ± 3.78 cd 50.59 ± 2.80d 13.48 ± 2.58c 0.00 ± 0.00c
KF-7-ENA5 86.88 ± 1.55d 12.62 ± 4.69 cd 50.10 ± 3.37d 10.90 ± 1.88c 0.00 ± 0.00c
  KF-7ΔASP3 89.53 ± 1.29 cd 5.89 ± 2.90d 41.76 ± 4.16d 15.05 ± 1.31bc 34.11 ± 2.72a
  KF-7ΔTOS8 95.74 ± 2.71bc 19.88 ± 3.72bc 67.85 ± 3.33bc 13.74 ± 1.91c 9.76 ± 1.88b
  KF-7ΔYOL162W 98.98 ± 1.86ab 57.75 ± 5.06a 85.36 ± 2.35a 29.66 ± 2.92a 1.36 ± 0.53c
  KF-7ΔYOR012W 90.97 ± 1.13 cd 18.56 ± 2.63c 61.66 ± 2.84c 19.48 ± 1.17b 14.66 ± 3.09b
Ethanol yield (g ethanol/g consumed glucose) KF-7 0.45 ± 0.01a 0.45 ± 0.01bc 0.47 ± 0.01a 0.50 ± 0.01a 0.42 ± 0.02ab
KF-7-CRZ1 0.41 ± 0.01b 0.46 ± 0.00ab 0.46 ± 0.01a 0.50 ± 0.00a 0.44 ± 0.02ab
KF-7-ENA5 0.44 ± 0.00a 0.48 ± 0.02a 0.46 ± 0.01a 0.50 ± 0.01a 0.45 ± 0.01a
  KF-7ΔASP3 0.44 ± 0.00a 0.47 ± 0.00ab 0.46 ± 0.01a 0.50 ± 0.00a 0.41 ± 0.00bc
  KF-7ΔTOS8 0.44 ± 0.02a 0.46 ± 0.01ab 0.47 ± 0.01a 0.49 ± 0.01a 0.41 ± 0.00bc
  KF-7ΔYOL162W 0.46 ± 0.01a 0.42 ± 0.01c 0.32 ± 0.02b 0.49 ± 0.01a 0.41 ± 0.01bc
  KF-7ΔYOR012W 0.45 ± 0.01a 0.46 ± 0.01ab 0.45 ± 0.02a 0.50 ± 0.01a 0.39 ± 0.01c
  1. The data in the table are those at the end of fermentation. Values indicate mean ± standard deviation of three biological replications. Values followed by different lowercase letters in the same column indicate significant differences at the level of P < 0.05 (Tukey-test) among strains. Same lowercase letters, no difference. KF-7-CRZ1: overexpression of TF Crz1p in KF-7; KF-7-ENA5: overexpression of ENA5 in KF-7; KF-7ΔASP3: Knockout ASP3 in KF-7; KF-7ΔTOS8: Knockout TOS8 in KF-7; KF-7ΔYOL162W: Knockout YOL162W in KF-7; KF-7ΔYOR012W: Knockout YOR012W in KF-7