|
Plasmids
|
|
Cas9-NAT
|
Ampr; Cas9; NAT1
|
[53]
|
|
pMEL13
|
Ampr; 2 μm origin, KanMX, gRNA-CAN1.Y
|
[54]
|
|
pMEL13-CRZ1
|
Ampr; 2 μm origin, KanMX, gRNA-CRZ1
|
This study
|
|
pMEL13-ENA5
|
Ampr; 2 μm origin, KanMX, gRNA-ENA5
|
This study
|
|
pMEL13-ASP3
|
Ampr; 2 μm origin, KanMX, gRNA-ASP3
|
This study
|
|
pMEL13-TOS8
|
Ampr; 2 μm origin, KanMX, gRNA-TOS8
|
This study
|
|
pMEL13-YOL162W
|
Ampr; 2 μm origin, KanMX, gRNA-YOL162W
|
This study
|
|
pMEL13-YOR012W
|
Ampr; 2 μm origin, KanMX, gRNA-YOR012W
|
This study
|
|
Strains
| | |
|
KF-7
|
Flocculating diploid industrial Saccharomyces cerevisiae strain
|
[55]
|
|
E-158
|
KF-7; Random mutagenesis and hybridization
|
[18]
|
|
KF-7-CRZ1
|
KF-7; Replacement of promoter PCRZ1 to PTEF1
|
This study
|
|
KF-7-ENA5
|
KF-7; Replacement of promoter PENA5 to PTEF1
|
This study
|
|
KF-7ΔASP3
|
KF-7; Knockout ASP3
|
This study
|
|
KF-7ΔTOS8
|
KF-7; Knockout TOS8
|
This study
|
|
KF-7ΔYOL162W
|
KF-7; Knockout YOL162W
|
This study
|
|
KF-7ΔYOR012W
|
KF-7; Knockout YOR012W
|
This study
|
|
E-158-CRZ1
|
E-158; Replacement of promoter PCRZ1 to PTEF1
|
This study
|
|
E-158-ENA5
|
E-158; Replacement of promoter PENA5 to PTEF1
|
This study
|
