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Table 4 Plasmids and strains

From: Screening novel genes by a comprehensive strategy to construct multiple stress-tolerant industrial Saccharomyces cerevisiae with prominent bioethanol production

Plasmids and strains Description References
Plasmids
 Cas9-NAT Ampr; Cas9; NAT1 [53]
 pMEL13 Ampr; 2 μm origin, KanMX, gRNA-CAN1.Y [54]
 pMEL13-CRZ1 Ampr; 2 μm origin, KanMX, gRNA-CRZ1 This study
 pMEL13-ENA5 Ampr; 2 μm origin, KanMX, gRNA-ENA5 This study
 pMEL13-ASP3 Ampr; 2 μm origin, KanMX, gRNA-ASP3 This study
 pMEL13-TOS8 Ampr; 2 μm origin, KanMX, gRNA-TOS8 This study
 pMEL13-YOL162W Ampr; 2 μm origin, KanMX, gRNA-YOL162W This study
 pMEL13-YOR012W Ampr; 2 μm origin, KanMX, gRNA-YOR012W This study
Strains   
 KF-7 Flocculating diploid industrial Saccharomyces cerevisiae strain [55]
 E-158 KF-7; Random mutagenesis and hybridization [18]
 KF-7-CRZ1 KF-7; Replacement of promoter PCRZ1 to PTEF1 This study
 KF-7-ENA5 KF-7; Replacement of promoter PENA5 to PTEF1 This study
 KF-7ΔASP3 KF-7; Knockout ASP3 This study
 KF-7ΔTOS8 KF-7; Knockout TOS8 This study
 KF-7ΔYOL162W KF-7; Knockout YOL162W This study
 KF-7ΔYOR012W KF-7; Knockout YOR012W This study
 E-158-CRZ1 E-158; Replacement of promoter PCRZ1 to PTEF1 This study
 E-158-ENA5 E-158; Replacement of promoter PENA5 to PTEF1 This study