Table 4 Plasmids and strains
Plasmids and strains | Description | References |
|---|---|---|
Plasmids | ||
 Cas9-NAT | Ampr; Cas9; NAT1 | [53] |
 pMEL13 | Ampr; 2 μm origin, KanMX, gRNA-CAN1.Y | [54] |
 pMEL13-CRZ1 | Ampr; 2 μm origin, KanMX, gRNA-CRZ1 | This study |
 pMEL13-ENA5 | Ampr; 2 μm origin, KanMX, gRNA-ENA5 | This study |
 pMEL13-ASP3 | Ampr; 2 μm origin, KanMX, gRNA-ASP3 | This study |
 pMEL13-TOS8 | Ampr; 2 μm origin, KanMX, gRNA-TOS8 | This study |
 pMEL13-YOL162W | Ampr; 2 μm origin, KanMX, gRNA-YOL162W | This study |
 pMEL13-YOR012W | Ampr; 2 μm origin, KanMX, gRNA-YOR012W | This study |
Strains | Â | Â |
 KF-7 | Flocculating diploid industrial Saccharomyces cerevisiae strain | [55] |
 E-158 | KF-7; Random mutagenesis and hybridization | [18] |
 KF-7-CRZ1 | KF-7; Replacement of promoter PCRZ1 to PTEF1 | This study |
 KF-7-ENA5 | KF-7; Replacement of promoter PENA5 to PTEF1 | This study |
 KF-7ΔASP3 | KF-7; Knockout ASP3 | This study |
 KF-7ΔTOS8 | KF-7; Knockout TOS8 | This study |
 KF-7ΔYOL162W | KF-7; Knockout YOL162W | This study |
 KF-7ΔYOR012W | KF-7; Knockout YOR012W | This study |
 E-158-CRZ1 | E-158; Replacement of promoter PCRZ1 to PTEF1 | This study |
 E-158-ENA5 | E-158; Replacement of promoter PENA5 to PTEF1 | This study |