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Fig. 3 | Biotechnology for Biofuels and Bioproducts

Fig. 3

From: Declining carbohydrate solubilization with increasing solids loading during fermentation of cellulosic feedstocks by Clostridium thermocellum: documentation and diagnostic tests

Fig. 3

Spent media inhibition tests. The effect of added spent media on net cumulative product formation (acetate + ethanol + formate + lactate in [mM]) during fermentation of 5 g/L cellobiose (panels A, B, C) or cellulose (panels D, E, F) for monocultures of Clostridium thermocellum. Panels B and E show the effect of spent media from corn stover fermentations by C. thermocellum at two different initial solid loadings, and panels C and F show the effect of spent media at two different senescent switchgrass loadings. Panels A and D show results for addition of spent media from a cellobiose (A) and cellulose (D) fermentations with carbohydrate utilization equal to that of 80 g/L corn stover solubilization (12.1 g/L cellobiose (CB) or cellulose (Av)). Individual controls (n = 1, dashed lines) with water in lieu of spent broth were included for each condition, whereas all other datapoints represent the average of triplicate bottle fermentations (n = 3). Error bars represent 1 standard deviation

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