Fig. 4
From: Bifunctional optogenetic switch for improving shikimic acid production in E. coli
Shikimic acid production. a Light controlled shikimic acid production pathway. Light controlled shikimic acid production pathway. GlC, glucose; Zmglf, glucose facilitator from Z. mobilis; PTS, phosphotransferase system; G6P, glucose 6-phosphate; Ru5P, ribulose-5-phosphate; PEP, phosphoenolpyruvate; PYR, pyruvate; aroBopt, codons optimized DHQ synthase; ydiB, quinate/shikimate dehydrogenase; aroGfbr, feedback-resistant mutants of DAHP synthase; E4P, erythrose-4-phosphate; aroK, shikimate kinase I; DAHP, 3-deoxy-D-arabino-heptulosonate-7-phosphate; QA, Quinic acid; DHQ, 3-dehydroquinic acid; DHS, 3-dehydroshikimic acid; SA, shikimate; S3P, shikimate-3-phosphate; AAA, aromatic amino acids. Gene knockouts were presented in grey color. b Screening the suitable chassis strain for shikimic acid production in a rich medium. c Design of TPAS and TPRS for shikimic acid production. d Evaluation of bifunctional optogenetic switch (S8) and individual components, TPRS (S6) and TPAS (S7) systems, for shikimic acid production. e Fed-batch fermentation using NBS minimal medium in a 5 L bioreactor. f Fed-batch fermentation using an enriched medium in a 5 L bioreactor. Values are shown as the mean ± s.d. (n = 3)