Table 1 Strains used in this study
From: Bifunctional optogenetic switch for improving shikimic acid production in E. coli
Strains/Plasmids | Relevant characteristics | Source |
|---|---|---|
Strains | Â | Â |
 K1 | E. coli MG1655 carrying pJ01-TetR | This study |
 TRU(N26/C27) | E. coli MG1655 carrying pJ01-TetR-26, Ptet-GFP | This study |
 TRU(N36/C37) | E. coli MG1655 carrying pJ01-TetR-36, Ptet-GFP | This study |
 TRU(N46/C47) | E. coli MG1655 carrying pJ01-TetR-46, Ptet-GFP | This study |
 TRU (N68/C69) | E. coli MG1655 carrying pJ01-TetR-68, Ptet-GFP | This study |
 TRU (N99/C100) | E. coli MG1655 carrying pJ01-TetR-99, Ptet-GFP | This study |
 TRU(N104/C105) | E. coli MG1655 carrying pJ01-TetR-104, Ptet-GFP | This study |
 TRU(N124/C125) | E. coli MG1655 carrying pJ01-TetR-124, Ptet-GFP | This study |
 TRU(N166/C167) | E. coli MG1655 carrying pJ01-TetR-166, Ptet-GFP | This study |
 TRU(N167/C168) | E. coli MG1655 carrying pJ01-TetR-167, Ptet-GFP | This study |
 TRU(N169/C170) | E. coli MG1655 carrying pJ01-TetR-169, Ptet-GFP | This study |
 TRU(N179/C180) | E. coli MG1655 carrying pJ01-TetR-179, Ptet-GFP | This study |
 XN | E. coli MG1655, ΔLacI | This study |
 TAU(N124/C125) | XN carrying pJ01-TetR-124, Ptet LacI-GFP | This study |
 TAU(N46/C47) | XN carrying pJ01-TetR-46, Ptet LacI-GFP | This study |
 TAU(N169/C170) | XN carrying pJ01-TetR-169, Ptet LacI-GFP | This study |
 TAU(N179/C180) | XN carrying pJ01-TetR-179, Ptet LacI-GFP | This study |
 TAU(N26/C27) | XN carrying pJ01-TetR-26, Ptet LacI-GFP | This study |
 TAU(N166/C167) | XN carrying pJ01-TetR-166, Ptet LacI-GFP | This study |
 TAU(N167/C168) | XN carrying pJ01-TetR-167, Ptet LacI-GFP | This study |
 TAU(N68/C69) | XN carrying pJ01-TetR-68, Ptet LacI-GFP | This study |
 PRU-118 | E. coli MG1655 carrying TEVp-118, Ptet-(TeF)GFP | This study |
 PRU-118-NW | E. coli MG1655 carrying TEVp-118-NW, Ptet-(TeF)GFP | This study |
 PAU-118 | E. coli MG1655 carrying TEVp-118, Ptet-GFP(LAA) | This study |
 PAU-118-NW | E. coli MG1655 carrying TEVp-118-NW, Ptet-GFP(LAA) | This study |
 TPRS | E. coli MG1655 carrying pJ01-TetR(166)-TEVp(118), Ptet-(TeF)GFP | This study |
 TPAS | XN carrying pJ01-TetR(166)-TEVp(118), Ptet-(TeF)LacI-GFP(DAS) | This study |
 S4 | E. coli MG1655, ΔptsHIcrr::Zmglf, ΔaroL::tktA, ΔaroK, PJ23119-GB | Lab stock |
 S5 | S4, ΔaroK::rpE, ΔyidB | This study |
 S6 | S5 carrying pJ01-TetR(166)-TEVp(118), NX1 | This study |
 S7 | S5 carrying pJ01-TetR(166)-TEVp(118), NX2 | This study |
 S8 | S5 carrying pJ01-TetR(166)-TEVp(118), NX3 | This study |
Plasmids | ||
 pJ01 | PJ23119 promoter, pMB1 ori, AmpR | Lab stock |
 pJ01-TetR-26 | pJ01-containing B0034RBS, TetR(N26)-VVD, VVD-TetR(C27) | This study |
 pJ01-TetR-36 | pJ01-containing B0034RBS, TetR(N36)-VVD, VVD-TetR(C37) | This study |
 pJ01-TetR-46 | pJ01-containing B0034RBS, TetR(N46)-VVD, VVD-TetR(C47) | This study |
 pJ01-TetR-68 | pJ01-containing B0034RBS, TetR(N68)-VVD, VVD-TetR(C69) | This study |
 pJ01-TetR-99 | pJ01-containing B0034RBS, TetR(N99)-VVD, VVD-TetR(C100) | This study |
 pJ01-TetR-102 | pJ01-containing B0034RBS, TetR(N104)-VVD, VVD-TetRC105) | This study |
 pJ01-TetR-124 | pJ01-containing B0034RBS, TetR(N124)-VVD, VVD-TetR(C125) | This study |
 pJ01-TetR-166 | pJ01-containing B0034RBS, TetR(N166)-VVD, VVD-TetR(C167) | This study |
 pJ01-TetR-167 | pJ01-containing B0034RBS, TetR(N167)-VVD, VVD-TetR(C168) | This study |
 pJ01-TetR-169 | pJ01-containing B0034RBS, TetR(N169)-VVD, VVD-TetR(C170) | This study |
 pJ01-TetR-179 | pJ01-containing B0034RBS, TetR(N179)-VVD, VVD-TetR(C180) | This study |
 pJ01-TetR | pJ01-containing B0034RBS, TetR(Full length) | This study |
 PTet-1 | Ptet, p15A ori, CmR, TetR | Lab stock |
 PTet-2 | Ptet, p15A ori, CmR | This study |
 Ptet-GFP | PTet-2 containing B0034RBS, GFP | This study |
 Ptet LacI-GFP | PTet-2 containing Ptet, B0034RBS, LacI, Ptrc, B0034RBS, GFP | This study |
 TEVp-118 | pJ01 containing Pj23119, B0034RBS, TEVp(N118)-VVD, VVD-TEVp(C180) | This study |
 TEVp-118-NW | pJ01 containing Pj23119, B0034RBS, VVD-TEVp(N118), VVD-TEVp(C180) | This study |
 Ptet-(TeF)GFP | PTet-2 containing Ptet, B0034RBS, (TeF)GFP | This study |
 Ptet-GFP(LAA) | PTet-2 containing Ptet, B0034RBS, GFP(LAA) | This study |
 pJ01-TetR(166)-TEVp(118) | pJ01 containing B0034RBS, TetR(N166)-VVD, VVD-TetR(C167), Pj23119-VVD-TEVp(N118), VVD-TEVp(C180) | This study |
 Ptet-(TeF)LacI-GFP(DAS) | PTet-2 containing Ptet, B0034RBS, (TeF)LacI, GFP fused with DAS tag | This study |
 pJ01-TEVp | pJ01 containing Pj23119, B0034RBS, TEVp(Full length) | This study |
 PJ01-GB | pJ01 containing Pj23119, B0034RBS, AroG, AroB | This study |
 NX-1 | PTet-2 containing Ptet, B0034RBS, (TeF)AroK | This study |
 NX-2 | PTet-2 containing Ptet, B0034RBS, (TeF)LacI, Ptrc, B0034RBS, AroG(DAS), AroB(DAS) | This study |
 NX-3 | PTet-2 containing Ptet, B0034RBS, (TeF)AroK, (TeF)LacI, Ptrc, B0034RBS, AroG(DAS), AroB(DAS) | This study |