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Table 1 Strains used in this study

From: Bifunctional optogenetic switch for improving shikimic acid production in E. coli

Strains/Plasmids Relevant characteristics Source
Strains   
 K1 E. coli MG1655 carrying pJ01-TetR This study
 TRU(N26/C27) E. coli MG1655 carrying pJ01-TetR-26, Ptet-GFP This study
 TRU(N36/C37) E. coli MG1655 carrying pJ01-TetR-36, Ptet-GFP This study
 TRU(N46/C47) E. coli MG1655 carrying pJ01-TetR-46, Ptet-GFP This study
 TRU (N68/C69) E. coli MG1655 carrying pJ01-TetR-68, Ptet-GFP This study
 TRU (N99/C100) E. coli MG1655 carrying pJ01-TetR-99, Ptet-GFP This study
 TRU(N104/C105) E. coli MG1655 carrying pJ01-TetR-104, Ptet-GFP This study
 TRU(N124/C125) E. coli MG1655 carrying pJ01-TetR-124, Ptet-GFP This study
 TRU(N166/C167) E. coli MG1655 carrying pJ01-TetR-166, Ptet-GFP This study
 TRU(N167/C168) E. coli MG1655 carrying pJ01-TetR-167, Ptet-GFP This study
 TRU(N169/C170) E. coli MG1655 carrying pJ01-TetR-169, Ptet-GFP This study
 TRU(N179/C180) E. coli MG1655 carrying pJ01-TetR-179, Ptet-GFP This study
 XN E. coli MG1655, ΔLacI This study
 TAU(N124/C125) XN carrying pJ01-TetR-124, Ptet LacI-GFP This study
 TAU(N46/C47) XN carrying pJ01-TetR-46, Ptet LacI-GFP This study
 TAU(N169/C170) XN carrying pJ01-TetR-169, Ptet LacI-GFP This study
 TAU(N179/C180) XN carrying pJ01-TetR-179, Ptet LacI-GFP This study
 TAU(N26/C27) XN carrying pJ01-TetR-26, Ptet LacI-GFP This study
 TAU(N166/C167) XN carrying pJ01-TetR-166, Ptet LacI-GFP This study
 TAU(N167/C168) XN carrying pJ01-TetR-167, Ptet LacI-GFP This study
 TAU(N68/C69) XN carrying pJ01-TetR-68, Ptet LacI-GFP This study
 PRU-118 E. coli MG1655 carrying TEVp-118, Ptet-(TeF)GFP This study
 PRU-118-NW E. coli MG1655 carrying TEVp-118-NW, Ptet-(TeF)GFP This study
 PAU-118 E. coli MG1655 carrying TEVp-118, Ptet-GFP(LAA) This study
 PAU-118-NW E. coli MG1655 carrying TEVp-118-NW, Ptet-GFP(LAA) This study
 TPRS E. coli MG1655 carrying pJ01-TetR(166)-TEVp(118), Ptet-(TeF)GFP This study
 TPAS XN carrying pJ01-TetR(166)-TEVp(118), Ptet-(TeF)LacI-GFP(DAS) This study
 S4 E. coli MG1655, ΔptsHIcrr::Zmglf, ΔaroL::tktA, ΔaroK, PJ23119-GB Lab stock
 S5 S4, ΔaroK::rpE, ΔyidB This study
 S6 S5 carrying pJ01-TetR(166)-TEVp(118), NX1 This study
 S7 S5 carrying pJ01-TetR(166)-TEVp(118), NX2 This study
 S8 S5 carrying pJ01-TetR(166)-TEVp(118), NX3 This study
Plasmids
 pJ01 PJ23119 promoter, pMB1 ori, AmpR Lab stock
 pJ01-TetR-26 pJ01-containing B0034RBS, TetR(N26)-VVD, VVD-TetR(C27) This study
 pJ01-TetR-36 pJ01-containing B0034RBS, TetR(N36)-VVD, VVD-TetR(C37) This study
 pJ01-TetR-46 pJ01-containing B0034RBS, TetR(N46)-VVD, VVD-TetR(C47) This study
 pJ01-TetR-68 pJ01-containing B0034RBS, TetR(N68)-VVD, VVD-TetR(C69) This study
 pJ01-TetR-99 pJ01-containing B0034RBS, TetR(N99)-VVD, VVD-TetR(C100) This study
 pJ01-TetR-102 pJ01-containing B0034RBS, TetR(N104)-VVD, VVD-TetRC105) This study
 pJ01-TetR-124 pJ01-containing B0034RBS, TetR(N124)-VVD, VVD-TetR(C125) This study
 pJ01-TetR-166 pJ01-containing B0034RBS, TetR(N166)-VVD, VVD-TetR(C167) This study
 pJ01-TetR-167 pJ01-containing B0034RBS, TetR(N167)-VVD, VVD-TetR(C168) This study
 pJ01-TetR-169 pJ01-containing B0034RBS, TetR(N169)-VVD, VVD-TetR(C170) This study
 pJ01-TetR-179 pJ01-containing B0034RBS, TetR(N179)-VVD, VVD-TetR(C180) This study
 pJ01-TetR pJ01-containing B0034RBS, TetR(Full length) This study
 PTet-1 Ptet, p15A ori, CmR, TetR Lab stock
 PTet-2 Ptet, p15A ori, CmR This study
 Ptet-GFP PTet-2 containing B0034RBS, GFP This study
 Ptet LacI-GFP PTet-2 containing Ptet, B0034RBS, LacI, Ptrc, B0034RBS, GFP This study
 TEVp-118 pJ01 containing Pj23119, B0034RBS, TEVp(N118)-VVD, VVD-TEVp(C180) This study
 TEVp-118-NW pJ01 containing Pj23119, B0034RBS, VVD-TEVp(N118), VVD-TEVp(C180) This study
 Ptet-(TeF)GFP PTet-2 containing Ptet, B0034RBS, (TeF)GFP This study
 Ptet-GFP(LAA) PTet-2 containing Ptet, B0034RBS, GFP(LAA) This study
 pJ01-TetR(166)-TEVp(118) pJ01 containing B0034RBS, TetR(N166)-VVD, VVD-TetR(C167), Pj23119-VVD-TEVp(N118), VVD-TEVp(C180) This study
 Ptet-(TeF)LacI-GFP(DAS) PTet-2 containing Ptet, B0034RBS, (TeF)LacI, GFP fused with DAS tag This study
 pJ01-TEVp pJ01 containing Pj23119, B0034RBS, TEVp(Full length) This study
 PJ01-GB pJ01 containing Pj23119, B0034RBS, AroG, AroB This study
 NX-1 PTet-2 containing Ptet, B0034RBS, (TeF)AroK This study
 NX-2 PTet-2 containing Ptet, B0034RBS, (TeF)LacI, Ptrc, B0034RBS, AroG(DAS), AroB(DAS) This study
 NX-3 PTet-2 containing Ptet, B0034RBS, (TeF)AroK, (TeF)LacI, Ptrc, B0034RBS, AroG(DAS), AroB(DAS) This study