Fig. 8

Effects of CRISPRi and CRISPRa of the selected genes on production of astaxanthin in E. coli AST-4AS. E. coli AST-4AS harboring pBbB2K-dCas9*-MCPSoxS and pTargetA-X was set as the control. CRISPRi for yadC, ygfI and rcsC; CRISPRa for rnb, envZ and recC. The data represent the means of three replicates, and error bars indicate standard deviations