Strain | Description | Source /reference |
---|---|---|
E. coli AST-4 | Astaxanthin producing strain, E. coli AST-2 ΔlppΔbamBΔuspEΔyggE | [17] |
E. coli AST-4A | Astaxanthin producing strain, E. coli AST-4 mutant after the adaptive laboratory evolution | This study |
E. coli AST-4AS | Astaxanthin producing strain, the shuffled strain of E. coli AST-4A after ep-WGS | This study |
E. coli AST-4ASΔyadC::P37-rnb | Astaxanthin producing strain, replacement of yadC with the rnb under the control of P37 promoter in E. coli AST-4AS | This study |
pSIM6 | pSC101 replicon, PL-gam-bet-exo, Ampr | [40] |
pBbB2K-dCas9*-MCPSoxS | CRISPRi/CRIPRa plasmid; pBbB2K-dCas9* containing the MCPSoxS sequences | [38] |
pTargetA | E. coli scRNA expression vector, BglBrick vector, Ptet promoter, Sper, pMB1 ori | [38] |
pZSKBP | constitute expression vector, pSC101 ori, P37 promoter, kanr, BglBrick, ePathBrick containing four isocaudamer (AvrII, NheI, SpeI, and XbaI) | [17] |