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Table 4 Strains and plasmids used in this study

From: Adaptive laboratory evolution and shuffling of Escherichia coli to enhance its tolerance and production of astaxanthin

Strain

Description

Source /reference

E. coli AST-4

Astaxanthin producing strain, E. coli AST-2 ΔlppΔbamBΔuspEΔyggE

[17]

E. coli AST-4A

Astaxanthin producing strain, E. coli AST-4 mutant after the adaptive laboratory evolution

This study

E. coli AST-4AS

Astaxanthin producing strain, the shuffled strain of E. coli AST-4A after ep-WGS

This study

E. coli AST-4ASΔyadC::P37-rnb

Astaxanthin producing strain, replacement of yadC with the rnb under the control of P37 promoter in E. coli AST-4AS

This study

pSIM6

pSC101 replicon, PL-gam-bet-exo, Ampr

[40]

pBbB2K-dCas9*-MCPSoxS

CRISPRi/CRIPRa plasmid; pBbB2K-dCas9* containing the MCPSoxS sequences

[38]

pTargetA

E. coli scRNA expression vector, BglBrick vector, Ptet promoter, Sper, pMB1 ori

[38]

pZSKBP

constitute expression vector, pSC101 ori, P37 promoter, kanr, BglBrick, ePathBrick containing four isocaudamer

(AvrII, NheI, SpeI, and XbaI)

[17]