Fig. 4From: Rational and evolutionary engineering of Saccharomyces cerevisiae for production of dicarboxylic acids from lignocellulosic biomass and exploring genetic mechanisms of the yeast tolerance to the biomass hydrolysateNext generation sequencing of the individual isolates evolved for tolerance to the SSL. a Schematic representation of the NGS data analysis and outcome. SNPs and indels were detected by reference-free pairwise comparison between each evolved isolate and the parental strain, performed with DiscoSnp +  + , followed by mapping of the variants to the reference genomes. CNVs were obtained by mapping to the Ethanol Red genome. b Number of mutations found per strain, split in new variants and loss of heterozygosity (LOH) events. Venn diagrams show overlaps between strains EV6_4 and EV9_5. c Schematic representation of the mutations found in some selected genes of interest, shared between strains. d Schematic representation of the changes found in the engineered xylose pathway genesBack to article page