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Fig. 4 | Biotechnology for Biofuels and Bioproducts

Fig. 4

From: Rational and evolutionary engineering of Saccharomyces cerevisiae for production of dicarboxylic acids from lignocellulosic biomass and exploring genetic mechanisms of the yeast tolerance to the biomass hydrolysate

Fig. 4

Next generation sequencing of the individual isolates evolved for tolerance to the SSL. a Schematic representation of the NGS data analysis and outcome. SNPs and indels were detected by reference-free pairwise comparison between each evolved isolate and the parental strain, performed with DiscoSnp +  + , followed by mapping of the variants to the reference genomes. CNVs were obtained by mapping to the Ethanol Red genome. b Number of mutations found per strain, split in new variants and loss of heterozygosity (LOH) events. Venn diagrams show overlaps between strains EV6_4 and EV9_5. c Schematic representation of the mutations found in some selected genes of interest, shared between strains. d Schematic representation of the changes found in the engineered xylose pathway genes

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