Fig. 3From: Simultaneous manipulation of multiple genes within a same regulatory stage for iterative evolution of Trichoderma reeseiManipulating Stage 2 to improve cellulase production. A FACS analysis of the transformant spores at 13 h post-induction or protoplasts prepared from the transformant mycelia at 72 h post-induction. The box indicated the protoplast sampling. B An initial screening of endoglucanase activity in the selected transformants on day 5. C and D. Production of endoglucanase (C) and extracellular protein concentration (D) of the transformantsBack to article page