Fig. 4

Manipulating Stage 3 to improve cellulase production. A FACS analysis of the protoplasts prepared from the transformant mycelia at 72 h post-cellulase induction. The box indicated the protoplast sampling. B An initial screening of endoglucanase activity in the selected transformants on day 5. C and D Production of endoglucanase (C) and extracellular protein concentration (D) of the transformants