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Table 2 The relative transcript abundance of transformed genes*

From: Simultaneous manipulation of multiple genes within a same regulatory stage for iterative evolution of Trichoderma reesei

Stage

Gene

Parental strain

Representative transformants

    

1: transcription

 

SUS4

1–2

1–3

1–13

1–25

1–31

ace3

1.0

2.9 ± 0.3

2.2 ± 0.2

3.6 ± 0.2

N.D

N.D

xyr1

1.0

13.5 ± 1.1

8.6 ± 0.6

7.6 ± 0.6

8.8 ± 1.1

9.5 ± 0.7

26163

1.0

2.9 ± 0.2

N.D

N.D

1.1 ± 0.1

5.8 ± 0.6

66966

1.0

10.4 ± 1.7

1.0 ± 0.2

17.0 ± 1.2

2.1 ± 0.3

N.D

27600

1.0

3.2 ± 0.1

N.D

10.5 ± 0.3

N.D

N.D

74765

1.0

N.D

8.3 ± 0.4

N.D

N.D

N.D

64608

1.0

N.D

N.D

N.D

N.D

1.0 ± 0.1

2: secretion

 

1–2/Δpyr4

2–3

2–4

2–13

2–18

2–29

sed1

1.0

0.3 ± 0.0

1.4 ± 0.1

0.4 ± 0.1

0.6 ± 0.1

0.2 ± 0.1

der1

1.0

1.5 ± 0.2

0.6 ± 0.1

0.8 ± 0.1

1.5 ± 0.2

0.9 ± 0.0

ych1

1.0

1.1 ± 0.1

1.5 ± 0.2

1.5 ± 0.1

0.4 ± 0.1

0.9 ± 0.1

ymr1

1.0

0.3 ± 0.1

0.3 ± 0.1

0.2 ± 0.0

0.5 ± 0.1

0.7 ± 0.2

och1-2

1.0

1.1 ± 0.2

1.2 ± 0.1

0.4 ± 0.1

0.3 ± 0.0

0.2 ± 0.0

pep4

1.0

0.2 ± 0.1

0.8 ± 0.1

0.8 ± 0.2

0.8 ± 0.1

0.7 ± 0.1

doa10

1.0

0.6 ± 0.1

0.8 ± 0.1

1.6 ± 0.3

0.9 ± 0.1

1.2 ± 0.2

yps1

1.0

0.3 ± 0.1

0.8 ± 0.1

0.7 ± 0.1

1.7 ± 0.2

1.3 ± 0.1

3: cell metabolism

 

2–3/Δpyr4

3–2

3–4

3–8

3–18

3–25

80621

1.0

1.1 ± 0.2

2.3 ± 0.2

1.1 ± 0.1

1.2 ± 0.1

0.9 ± 0.2

52055

1.0

2.1 ± 0.3

0.9 ± 0.1

1.3 ± 0.2

1.1 ± 0.1

1.2 ± 0.0

53567

1.0

2.0 ± 0.2

1.3 ± 0.1

2.9 ± 0.3

2.3 ± 0.1

1.2 ± 0.1

75769

1.0

2.3 ± 02

1.1 ± 0.1

5.0 ± 0.3

2.4 ± 0.2

1.3 ± 0.1

72685

1.0

8.6 ± 0.2

3.0 ± 0.2

4.0 ± 0.2

4.1 ± 0.4

6.5 ± 0.2

73903

1.0

2.4 ± 0.3

1.1 ± 0.0

2.7 ± 0.1

2.6 ± 0.2

1.6 ± 0.1

57940

1.0

2.9 ± 0.1

0.7 ± 0.2

0.5 ± 0.1

0.8 ± 0.1

0.9 ± 0.0

  1. *All experiments were performed in three independent replicates
  2. N.D. not determined