Response mechanisms of Saccharomyces cerevisiae to the stress factors present in lignocellulose hydrolysate and strategies for constructing robust strains

Li, Bo; Liu, Nan; Zhao, Xuebing

doi:10.1186/s13068-022-02127-9

Biotechnology for Biofuels and Bioproducts

Table 2 Reported target genes in improving S. cerevisiae resistance to stress factors

From: Response mechanisms of Saccharomyces cerevisiae to the stress factors present in lignocellulose hydrolysate and strategies for constructing robust strains

Target gene	Function	Location	Regulation	Stress factor	Refs.
CCW12	Cell wall mannoprotein	CWB	Overexpression	Acetic acid	[36]
FPS1	Aquaglyceroporin	CMB	Dephosphorylation	Acetic acid	[110]
HOG1	Mitogen-activated protein kinase	CMB	Activation/overexpression	Acetic acid and osmostress	[110, 111]
ADY2	Acetate transporter	CMB	Deletion	Acetic acid and ROS	[105]
ATR1	Multidrug efflux pump of the major facilitator superfamily	CMB	Overexpression	Coniferyl aldehyde, ferulic acid, and isoeugenol	[112]
AZR1	Plasma membrane transporter	CMB	Overexpression	Acetic acid	[113]
FLR1	Plasma membrane transporter of the major facilitator superfamily	CMB	Overexpression	Coniferyl aldehyde, ferulic acid, and isoeugenol	[112]
PDR5	Plasma membrane ATP-binding cassette (ABC) transporter	CMB	Overexpression	Vanillin	[40]
YOR1	Plasma membrane ATP-binding cassette (ABC) transporter	CMB	Overexpression	Vanillin	[40]
SNQ2	Plasma membrane ATP-binding cassette (ABC) transporter	CMB	Overexpression	Vanillin	[40]
PMA1	Plasma membrane P2-type H+-ATPase	CMB	Overexpression	Organic acids and ROS	[114]
KAR2	Endoplasmic reticulum chaperone BiP	CMB	Overexpression	Vanillin	[115]
ACS2	Acetyl-coA synthetase	BMM	Overexpression	Acetic acid	[116]
ADE1/13/17	ADEnine	BMM	Overexpression	Acetic acid	[100]
BDH2	Putative medium-chain alcohol dehydrogenase/reductases	BMM	Overexpression	Vanillin	[101]
ADH1/6/7	Alcohol dehydrogenase	BMM	Overexpression	Furfural, HMF, and vanillin	[117,118,119]
ALD6/7	Aldehyde dehydrogenase	BMM	Overexpression	Furfural and HMF	[120,121,122]
GLR1	Glutathione oxidoreductase	BMM	Overexpression	Furfural	[123]
PAD1	Phenylacrylic acid decarboxylase	BMM	Overexpression	Phenylacrylic acids	[124]
PRS3	Phosphoribosyl pyrophosphate synthetase	BMM	Overexpression	Acetic acid	[11, 39]
PHO13	p-Nitrophenylphosphatase	BMM	Deletion	Formic, acetic, levulinic acids, and furfural	[125, 126]
RCK1	Protein kinase	BMM	Overexpression	Acetic acid and oxidative stress	[38]
SFA1	Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase	BMM	Overexpression	Acetic acid	[35]
WHI2	Cytoplasmic globular scaffold protein	BMM	Overexpression	Acetic acid	[127]
DBP2	ATP-dependent RNA helicase of the DEAD-box protein family	BMM	Overexpression	Vanillin	[40]
RPE1	Ribulose 5-phosphate epimerase	BMM	Overexpression	Furfural	[128]
TAL1	Transaldolase	BMM	Overexpression	Furfural	[117, 125]
TKL1	Transketolase	BMM	Overexpression	Furfural	[128]
GND1	6-Phosphogluconate dehydrogenase	BMM/IRH	Overexpression	Furfural	[128]
GSH1/2	Gamma glutamylcysteine synthetase	BMM/IRH	Overexpression	Oxidative stress, furfural, and HMF	[129]
IDP1	Isocitrate dehydrogenase	BMM/IRH	Overexpression	Furfural	[123]
ZWF1	Glucose-6-phosphate dehydrogenase	BMM/IRH	Overexpression	Furfural	[123, 128]
SET5	Methyltransferase	IRH	Overexpression	Acetic acid and ROS	[106]
JJJ1	Co-chaperone that stimulates the ATPase activity of Ssa1p	IRH	Deletion	Acetic acid and ROS	[107]
LacA	Laccase	-	Heterologous expression	Vanillin	[115]

These genes are mainly involved in cell wall barrier (CWB), cell membrane barrier (CMB), basic metabolism maintenance (BMM), and intracellular redox homeostasis (IRH)

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ISSN: 2731-3654

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