Fig. 2

A comparison of three xylanases using the PACER assay. A–C Composites were incubated in 0.01 mg/ mL CmXyn10B, 0.005 mg/mL NpXyn11A, 0.009 mg/mL TfXyn11A, or buffer only, at pH 5.0, 40 °C and 300 rpm for 1–6 h. A Representative cellulose/azo-xylan composites dried after treatment (Additional file 1: Fig. S5). B Average intensity values of dried cellulose/azo-xylan composites per segment. C Absorbance of the reaction solution at 590 nm recovered after composite incubation; statistically significant pairwise differences between CmXyn10B and NpXyn11A, or TfXyn11A and NpXyn11A are indicated by an asterisk (two-tailed t-test; p ≤ 0.05). For all experiments, n ≥ 4; errors bars correspond to standard deviation of mean