Table 1 Properties of enzymes used in this study
From: PACER: a novel 3D plant cell wall model for the analysis of non-catalytic and enzymatic responses
Enzyme | Organism | CAZY number | Molecular mass (kDa) | CBM | UNIPROT Accession number | Specific activity (U/mg)* |
|---|---|---|---|---|---|---|
CmXyn10B | Cellvibrio mixtus | GH10 | 41.7 | – | O68541 | 7.32 |
NpXyn11A | Neocallimastix patriciarum | GH11 | 25.8 | – | P29127 | 14.2 |
TfXyn11A | Thermobifida fusca | GH11 | 31.9 | CBM2b | Q47QL8 | 8.06 |
Pm25 (WT) | Unclassified; from termite gut metagenome | GH10 | 83.9 | CBM4 | S0DFK9 | 0.29 |
Pm25 Y213A Y378A (M5) | 83.8 | CBM4 | 0.29 | |||
Pm25∆CBMs (M6) | 48.8 | – | 0.49 | |||
Pm25 E546A (M1) | 83.9 | CBM4 | 0.01 |