Fig. 5
Hydrocarbons production by dual synthesis pathways in C. glutamicum. a Secretive expression of fatty acid decarboxylase OleTMC in C. glutamicum HW4. b Hydrocarbons production by dual synthesis pathways in C. glutamicum. HW5 indicates C. glutamicum HW4 with the secretive expression of oleTMC through Sec pathway and HW6 indicates C. glutamicum HW4 with the secretive expression of oleTMC through Tat pathway. Fermentation was carried out in shake flask, 30 °C, 200 rpm. pH was maintained at 7.0 by adding 5 M NaOH. c Improved hydrocarbons production by optimizing the culture conditions. Fermentation was carried out in a 1-L fermentor. The medium was mentioned in “Materials and methods”. The basic fermentation conditions were 30 °C, pH 7 and 600 rpm. When one of the fermentation parameters was changed for optimization, the others remained the same