Table 1 Strains and plasmids used in the study
From: Highly efficient biosynthesis of β-caryophyllene with a new sesquiterpene synthase from tobacco
Name | Relevant genotype/property | Source |
|---|---|---|
Strains | ||
E. coli BL21(DE3) | F− ompT hsdS(rB−mB−) gal dcm (DE3) | TransGen Biotec |
E. coli Trans5α | F−φ80d lac ZΔM15 Δ(lacZYA-argF) U169 end A1 recA1 hsdR17(rk−, mk+) supE44λ- thi-1 gyrA96 relA1 phoA | TransGen Biotec |
CM2 | BL21(DE3)::Trc-Low | [36] |
CAR0 | BL21(DE3)/pET-tps7 | This study |
CAR1 | BL21(DE3)/pYJM14, p-miT7 | This study |
CAR2 | CM2/p-miT7 | This study |
plasmids | ||
pET-28a( +) | oripBR322 lacIq T7p, Kanr | Novagen |
pEASY-tps7 | pEASY-Blunt derivative carrying gene tps7, T7 promoter, AmpR, KanR | Laboratory preserved |
pYJM14 | pTrcHis2B derivative carrying genes MVK, PMK, MVE, and IDI, Trc promoter, AmpR | [33] |
pACY-mvaE-mvaS | pACYCDuet-1 derivative carrying genes mvaE and mvaS, T7 promoter, CmR | [33] |
p-miT7 | pACYCDuet-1 derivative carrying genes mvaE, mvaS, ispA and tps7, T7 promoter, CmR | This study |
p-iT7 | pACYCDuet-1 derivative carrying genes ispA and tps7, T7 promoter, CmR | This study |
pET-tps7 | pET-28a( +) derivative carrying gene tps7, T7 promoter, KanR | This study |