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Table 1 Conversion of substrates to lactic acid by Caldicellulosiruptor BluCon L60

From: Direct conversion of cellulose to l-lactic acid by a novel thermophilic Caldicellulosiruptor strain

Substrate Substrate concentration in the culture Lactic acid produced Lactic acid ratio in fermentation products Lactic acid yield. C5/C6-sugars conversion to lactic acid LAE yield. C5/C6-sugars conversion to lactate + acetate + ethanol
  g (dry) / liter culture g/l wt% % of maximum % of maximum
Microcrystalline cellulose Avicel 18.3 16.8 ± 0.5 92.4 ± 0.4 85.7 ± 2.7 96.9 ± 2.9
  27.4 24.6 ± 0,2 93.0 ± 0.2 83.7 ± 0.6 93.6 ± 0.5
Microcrystalline cellulose Jelucel OF30 18.0 16.6 ± 0.2 92.6 ± 0.2 85.1 ± 0.9 95.6 ± 1.2
  27.0 23.3 ± 0.3 93.5 ± 0.0 79.9 ± 1.1 88.5 ± 1.3
Filter paper (Whatman #1) 18.5 17.1 ± 0.2 92.9 ± 0.1 87.1 ± 1.0 97.6 ± 1.0
  27.8 24.5 ± 0.2 92.9 ± 0.2 83.2 ± 0.8 93.1 ± 0.8
Newspaper 18.4 8.1 ± 0,1 89.9 ± 0.2 58.9 ± 0.8 69.2 ± 1.1
  27.6 10.9 ± 1.0 87.8 ± 4.5 52.6 ± 5.1 64.4 ± 1.5
Cardboard (brown) 18.3 9.3 ± 0.4 88.4 ± 0.1 62.3 ± 3.0 75.1 ± 3.7
  27.4 10.4 ± 0.7 87.6 ± 0.5 46.5 ± 3.0 56.7 ± 3.4
Recycled paper 18.3 8.3 ± 0.2 89.0 ± 0.3 63.2 ± 1.1 75.4 ± 1.2
  27.4 11.9 ± 0.3 90.1 ± 0.2 60.3 ± 1.4 70.7 ± 1.6
Xylan from beechwood 18.1 13.6 ± 0.9 88.5 ± 0.2 73.1 ± 4.6 88.7 ± 5.6
  27.2 18.7 ± 0.6 89.1 ± 0.6 67.2 ± 2.0 80.6 ± 3.2
Starch (soluble) 17.2 14.4 ± 0.9 91.2 ± 0.6 75.1 ± 4.7 86.6 ± 5.7
  25.9 23.2 ± 0.3 93.0 ± 0.3 80.9 ± 0.9 90.4 ± 0.8
Wheat straw (not pretreated) 18.1 3.1 ± 0.2 75.2 ± 1.0 26.3 ± 1.8 39.6 ± 2.0
  27.1 4.4 ± 0.1 77.7 ± 0.1 24.3 ± 0.6 35.0 ± 0.8
Wheat straw (6% NaOH-refiner) 21.4 8.8 ± 0.6 79.4 ± 4.7 49.3 ± 3.5 71.0 ± 1.3
  1. Substrate concentrations between 18 and 80 g/l were applied and a CaCO3 concentration of 50 g/l was added as buffer. Cultures were grown in flasks at 70 °C without shaking for a total time of 14 days to make sure that fermentation was performed until completion