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Table 1 Conversion of substrates to lactic acid by Caldicellulosiruptor BluCon L60

From: Direct conversion of cellulose to l-lactic acid by a novel thermophilic Caldicellulosiruptor strain

Substrate

Substrate concentration in the culture

Lactic acid produced

Lactic acid ratio in fermentation products

Lactic acid yield. C5/C6-sugars conversion to lactic acid

LAE yield. C5/C6-sugars conversion to lactate + acetate + ethanol

 

g (dry) / liter culture

g/l

wt%

% of maximum

% of maximum

Microcrystalline cellulose Avicel

18.3

16.8 ± 0.5

92.4 ± 0.4

85.7 ± 2.7

96.9 ± 2.9

 

27.4

24.6 ± 0,2

93.0 ± 0.2

83.7 ± 0.6

93.6 ± 0.5

Microcrystalline cellulose Jelucel OF30

18.0

16.6 ± 0.2

92.6 ± 0.2

85.1 ± 0.9

95.6 ± 1.2

 

27.0

23.3 ± 0.3

93.5 ± 0.0

79.9 ± 1.1

88.5 ± 1.3

Filter paper (Whatman #1)

18.5

17.1 ± 0.2

92.9 ± 0.1

87.1 ± 1.0

97.6 ± 1.0

 

27.8

24.5 ± 0.2

92.9 ± 0.2

83.2 ± 0.8

93.1 ± 0.8

Newspaper

18.4

8.1 ± 0,1

89.9 ± 0.2

58.9 ± 0.8

69.2 ± 1.1

 

27.6

10.9 ± 1.0

87.8 ± 4.5

52.6 ± 5.1

64.4 ± 1.5

Cardboard (brown)

18.3

9.3 ± 0.4

88.4 ± 0.1

62.3 ± 3.0

75.1 ± 3.7

 

27.4

10.4 ± 0.7

87.6 ± 0.5

46.5 ± 3.0

56.7 ± 3.4

Recycled paper

18.3

8.3 ± 0.2

89.0 ± 0.3

63.2 ± 1.1

75.4 ± 1.2

 

27.4

11.9 ± 0.3

90.1 ± 0.2

60.3 ± 1.4

70.7 ± 1.6

Xylan from beechwood

18.1

13.6 ± 0.9

88.5 ± 0.2

73.1 ± 4.6

88.7 ± 5.6

 

27.2

18.7 ± 0.6

89.1 ± 0.6

67.2 ± 2.0

80.6 ± 3.2

Starch (soluble)

17.2

14.4 ± 0.9

91.2 ± 0.6

75.1 ± 4.7

86.6 ± 5.7

 

25.9

23.2 ± 0.3

93.0 ± 0.3

80.9 ± 0.9

90.4 ± 0.8

Wheat straw (not pretreated)

18.1

3.1 ± 0.2

75.2 ± 1.0

26.3 ± 1.8

39.6 ± 2.0

 

27.1

4.4 ± 0.1

77.7 ± 0.1

24.3 ± 0.6

35.0 ± 0.8

Wheat straw (6% NaOH-refiner)

21.4

8.8 ± 0.6

79.4 ± 4.7

49.3 ± 3.5

71.0 ± 1.3

  1. Substrate concentrations between 18 and 80 g/l were applied and a CaCO3 concentration of 50 g/l was added as buffer. Cultures were grown in flasks at 70 °C without shaking for a total time of 14 days to make sure that fermentation was performed until completion