Fig. 1
GA performance in alkaline conditions and the whole screening process of PobA mutants. A The reaction mixture of GA and NaHCO3. GA concentrations in tube 1–12 were 0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.5 g/L, respectively. NaHCO3 concentration in the tube was 1 M. B The full wavelength (340–820 nm) scan results of the mixture. C The linear relationship of GA concentration and the optical density at 640 nm. D The whole screening process for screening high activity PobA mutants