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Fig. 4 | Biotechnology for Biofuels and Bioproducts

Fig. 4

From: A high-throughput visual screening method for p-hydroxybenzoate hydroxylase to increase phenolic compounds biosynthesis

Fig. 4

The de novo biosynthetic pathway of GA and pyrogallol. Black-colored arrows indicate the native pathways in E. coli; blue colored arrow indicates the heterologous steps; yellow-colored arrow indicates side-reaction step. PEP, phosphoenolpyruvate; E4P, D-erythrose 4-phosphate; DAHP, 3-deoxy-D-arabinoheptulosonate 7-phosphate; DHS 3-dehydroshikimate; 3,4-DHBA, 3,4-dihydroxybenzoic acid; GA, gallic acid; PpsA, phosphoenolpyruvate synthetase; TktA, transketolase; AroG, 2-dehydro-3-deoxyphosphoheptonate aldolase; AroD, 3-dehydroquinate dehydratase; AroE, shikimate dehydrogenase; YdiB, quinate/shikimate dehydrogenase; AroZ, 3-dehydroshikimate dehydratase; AroL, shikimate kinase II; UbiC, chorismate lyase; PobA mutant, p-hydroxybenzoate hydroxylase with mutations; PDC, 3,4-dihydroxybenzoic acid decarboxylase

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